2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00009002 | hint-1 | F21C3.3 | Caenorhabditis elegans |
| WBGene00016150 | hint-3 | C26F1.7 | Caenorhabditis elegans |
| WormBase ID | WBStrain00055400 | CGC Received | 2023-08-02 |
| Genotype | hint-1(sy1567) I; hint-3(sy2026) V. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | PS9997 |
| Outcrossed | x0 | Remark | Superficially wild type. Penetrance lethal with lots of unhatched eggs and slow growth. CRISPR/Cas9 engineered STOP-IN null mutant of hint-3 into hint-1(sy1567) stop-in mutant. Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). Left flanking sequence: catgttttacaggATGACGTCAATGCATACTTCTGT. Right flanking sequence: TAACGGATGCAAGTTTTGTGACATTGTCAAAAATA. Inserted sequence between the two-flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TCAATGCATACTTCTGTTAA. Method Reference: Wang H, et al. G3 (Bethesda). 2018 Nov 6;8(11):3607-3616. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00009002 | hint-1 | F21C3.3 | Caenorhabditis elegans |
| WBGene00016150 | hint-3 | C26F1.7 | Caenorhabditis elegans |
