3 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00005016 | sqt-1 | B0491.2 | Caenorhabditis elegans |
| WBGene00006537 | tbb-2 | C36E8.5 | Caenorhabditis elegans |
| WBGene00219689 | linc-41 | F36A4.22 | Caenorhabditis elegans |
| WormBase ID | WBStrain00055040 | CGC Received | 2022-06-22 |
| Genotype | linc-41(ot1231[linc-41p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) IV. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | OH17938 |
| Outcrossed | x0 | Remark | The Null Transcriptional Reporter (NuTR) cassette was used to remove the linc-41 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. GFP expression is seen in the male somatic gonad, spermatheca of hermaphrodites, as well as several other tissues in the head. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00005016 | sqt-1 | B0491.2 | Caenorhabditis elegans |
| WBGene00006537 | tbb-2 | C36E8.5 | Caenorhabditis elegans |
| WBGene00219689 | linc-41 | F36A4.22 | Caenorhabditis elegans |
