WormMine: Strain WBStrain00060125

• WormBase ID: WBStrain00060125
• CGC Received: 2024-05-31
• Genotype: fbf-1(ok91) fbf-2(q1023[*q973])/mIn1[mIs14 dpy-10(e128)] II.
• Laboratory: JK
• Mutagen: CRISPR
• Name: JK5986
• Outcrossed: x2
• Remark: q1023 is an engineered Y479A point mutation in the R7/R8 loop of 3xFLAG-tagged FBF-2. Derived by modification of parental strain JK5935 fbf-2(q945[3xFLAG::fbf-2]) II. Pick GFP+ t maintain. Homozygous sterile (Mog) mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok91 q1023 homozygotes (sterile Mog). Pick WT dim GFP and check for correct segregation of progeny to maintain. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520.
• Species: Caenorhabditis elegans

3 Alleles

Public Name
q1023
ok91
e128

1 Data Sets

• WormBaseAcedbConverter

3 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001072	dpy-10	T14B4.7	Caenorhabditis elegans
WBGene00001401	fbf-1	H12I13.4	Caenorhabditis elegans
WBGene00001402	fbf-2	F21H12.5	Caenorhabditis elegans