2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000254 | bli-4 | K04F10.4 | Caenorhabditis elegans |
| WBGene00001595 | gld-1 | T23G11.3 | Caenorhabditis elegans |
| WormBase ID | WBStrain00060132 | CGC Received | 2024-05-31 |
| Genotype | gld-1(q1271[*q1242]) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | Laboratory | JK |
| Made By | WBPerson123 | Mutagen | CRISPR |
| Name | JK6602 | Outcrossed | x2 |
| Remark | Engineered TGT to ACA substitutions in FBEa1 and FBEb of the endogenous gld-1 locus with a downstream G to C substitution to facilitate screening by restriction digest. Pick GFP+ to maintain. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP q1271 homozygotes (sterile Mog). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP+ and check for correct segregation of progeny to maintain. Derived by modification of gld-1(q1242) homozygotes from parental strain JK6540. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. | Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000254 | bli-4 | K04F10.4 | Caenorhabditis elegans |
| WBGene00001595 | gld-1 | T23G11.3 | Caenorhabditis elegans |
