WormMine

WS297

Intermine data mining platform for C. elegans and related nematodes

Strain :

WormBase ID  WBStrain00062667 CGC Received  2025-02-20
Genotype  col-75(wrd349[col-75::mNG::3xFLAG]) II. Laboratory  CGC
Made By  WBPerson6656 Mutagen  Crispr/Cas9
Name  JDW850 Outcrossed  x2
Remark  Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-75 locus by CRISPR. Allele obtained using plasmid-based unc-119 selection in a temperature-sensitive unc-119(kst33) III background The unc-119(+) cassette was removed through Cre-mediated excision and the unc-119(kst33) allele was removed by outcrossing. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Species  Caenorhabditis elegans

0 Alleles

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000651 col-75 K03H9.2 Caenorhabditis elegans