1 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00000651 | col-75 | K03H9.2 | Caenorhabditis elegans |
WormBase ID | WBStrain00062667 | CGC Received | 2025-02-20 |
Genotype | col-75(wrd349[col-75::mNG::3xFLAG]) II. | Laboratory | CGC |
Made By | WBPerson6656 | Mutagen | Crispr/Cas9 |
Name | JDW850 | Outcrossed | x2 |
Remark | Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-75 locus by CRISPR. Allele obtained using plasmid-based unc-119 selection in a temperature-sensitive unc-119(kst33) III background The unc-119(+) cassette was removed through Cre-mediated excision and the unc-119(kst33) allele was removed by outcrossing. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. | Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00000651 | col-75 | K03H9.2 | Caenorhabditis elegans |