3 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00001072 | dpy-10 | T14B4.7 | Caenorhabditis elegans |
WBGene00001401 | fbf-1 | H12I13.4 | Caenorhabditis elegans |
WBGene00001402 | fbf-2 | F21H12.5 | Caenorhabditis elegans |
WormBase ID | WBStrain00062696 | CGC Received | 2024-07-08 |
Genotype | fbf-1(q1251) fbf-2(q738)/mIn1 [mIs14 dpy-10(e128)] II. | Laboratory | CGC |
Mutagen | Crispr/Cas9 | Name | JK6548 |
Outcrossed | x2 | Remark | Pick wild-type GFP+ to maintain. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (Mog). Pick WT dim GFP and check for correct segregation of progeny to maintain. q1251 is an engineered H324A point mutation in FBF-1 derived by modification of parental strain JK3101. |
Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00001072 | dpy-10 | T14B4.7 | Caenorhabditis elegans |
WBGene00001401 | fbf-1 | H12I13.4 | Caenorhabditis elegans |
WBGene00001402 | fbf-2 | F21H12.5 | Caenorhabditis elegans |