2 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00001072 | dpy-10 | T14B4.7 | Caenorhabditis elegans |
WBGene00001402 | fbf-2 | F21H12.5 | Caenorhabditis elegans |
WormBase ID | WBStrain00062697 | CGC Received | 2024-07-08 |
Genotype | fbf-2(q1264[*q1011])/mIn1 [mIs14 dpy-10(e128)] II. | Laboratory | CGC |
Mutagen | Crispr/Cas9 | Name | JK6550 |
Outcrossed | x12 | Remark | Pick wild-type GFP+ to maintain. 3xFlag tag inserted into endogenous fbf-2 locus with engineered (H326A, Y479A) substitutions. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-2 homozygotes (sterile?). Pick WT dim GFP and check for correct segregation of progeny to maintain. |
Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00001072 | dpy-10 | T14B4.7 | Caenorhabditis elegans |
WBGene00001402 | fbf-2 | F21H12.5 | Caenorhabditis elegans |