3 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001072 | dpy-10 | T14B4.7 | Caenorhabditis elegans |
| WBGene00001401 | fbf-1 | H12I13.4 | Caenorhabditis elegans |
| WBGene00001402 | fbf-2 | F21H12.5 | Caenorhabditis elegans |
| WormBase ID | WBStrain00062690 | CGC Received | 2024-07-08 |
| Genotype | fbf-1(q1227) fbf-2(q945[3xFLAG::fbf-2])/mIn1 [mIs14 dpy-10(e128)] II. | Laboratory | CGC |
| Mutagen | Crispr/Cas9 | Name | JK6509 |
| Outcrossed | x6 | Remark | Pick wild-type GFP+ to maintain. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes. fbf-1(q1227) fbf-2(q945[3xFLAG::fbf-2]) homozygotes are partially sterile, ~50% make excess sperm and delay oogenesis resulting in delayed egg laying when compared to wild-type animals. Pick WT dim GFP and check for correct segregation of progeny to maintain. q1227 is an engineered Y477A point mutation in FBF-1 derived by modification of parental strain JK5810. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001072 | dpy-10 | T14B4.7 | Caenorhabditis elegans |
| WBGene00001401 | fbf-1 | H12I13.4 | Caenorhabditis elegans |
| WBGene00001402 | fbf-2 | F21H12.5 | Caenorhabditis elegans |
