2 Genes
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00000898 | daf-2 | Y55D5A.5 | Caenorhabditis elegans |
WBGene00002162 | isp-1 | F42G8.12 | Caenorhabditis elegans |
WormBase ID | WBStrain00062800 | CGC Received | 2024-05-23 |
Genotype | daf-2(e1370) III; isp-1(qm150) IV/nT1[qIs51] (IV;V). | Laboratory | CGC |
Made By | WBPerson12805 | Name | NOB142 |
Outcrossed | x3 | Remark | Maintain at 15-20C: will form dauers at higher temps. Slow growing. Pick GFP+ to maintain. Heterozygotes are GFP+ and segregate WT GFP+ hets, arrested nT1[qIs51] aneuploids, and non-GFP daf-2(e1370); isp-1(qm150) homozygotes (sterile). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. [NOTE: Originally published daf-2(e1370); isp-1(qm150) strain MQ876 was found to not actually contain the daf-2(e1370) mutation. This strain was made to study the double mutant and revealed that daf-2(e1370); isp-1(qm150) homozygotes are inviable/sterile.] Reference: Cooper et al. In preparation. |
Species | Caenorhabditis elegans |
WormBase Gene ID | Gene Name | Sequence Name | Organism |
---|---|---|---|
WBGene00000898 | daf-2 | Y55D5A.5 | Caenorhabditis elegans |
WBGene00002162 | isp-1 | F42G8.12 | Caenorhabditis elegans |