| WormBase ID | WBStrain00007373 | CGC Received | 2008-01-28 |
| Genotype | aph-1(or28)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(ec56) IV; lin-2(e1309) X. | Laboratory | CGC |
| Made By | WBPerson75 | Mutagen | EMS |
| Name | EU1514 | Outcrossed | x1 |
| Remark | or28 is a non-conditional maternal-effect embryonic lethal mutant ; the anterior pharynx is absent. and embryonic lethality is 100% penetrant. Pick single WT heterozygous hermaphrodites to maintain strain; these hets fill up with a mix of dead embryos and larvae [11/16 dead embryos due to aneuploidy from translocation segregation and lethal homozygous hT2; vulvaless due to homozygous lin-2(e1309) mutation; about 10% of lin-2(e1309) worms are leaky and lay eggs/can be mated into]. The homozygous or28 hermaphrodites fill up with dead embryos. The lin-2 background helps to score embryonic lethality for both heterozygotes and or28 homozygotes. Strain produces a lot of males due to him-8(ec56). or28 is a G to D mis-sense mutation at amino acid position 123. | Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000147 | aph-1 | VF36H2L.1 | Caenorhabditis elegans |
| WBGene00000254 | bli-4 | K04F10.4 | Caenorhabditis elegans |
| WBGene00001867 | him-8 | T07G12.12 | Caenorhabditis elegans |
| WBGene00002991 | lin-2 | F17E5.1 | Caenorhabditis elegans |
