1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00012484 | car-1 | Y18D10A.17 | Caenorhabditis elegans |
| Primary Identifier | Expr4815 | Remark | Depletion of CAR-1 did not affect P-granule formation or distribution, which indicated that CAR-1 is not necessary for either of these events. Particles were not detected in car-1(RNAi) embryos, which confirmed the specificity of the localization. Picture: Figure 2. |
| Subcellular Localization | CAR-1 localized to cytoplasmic particles whose size and distribution varied during the early embryonic divisions. From the latter half of the first division onward, CAR-1 localized prominently to large particles that were similar in size and distribution to P-granules. By performing immunofluorescence in a strain expressing GFP:PGL-1, authors confirmed that a subset of CAR-1 colocalizes with PGL-1 to P-granules. A more detailed comparison revealed a dynamic nature to the particulate localization of CAR-1 and PGL-1. In embryos in which the female pronucleus was completing meiosis, both proteins localized to numerous small particles that were distributed throughout the embryo. However, although particles that contained the two markers were juxtaposed, they were not coincident. By metaphase of the first mitotic division, most particulate CAR-1 colocalized with PGL-1 in large P-granules in the embryo posterior. However, a few smaller particles containing CAR-1, but not PGL-1, remained scattered throughout the embryo. In two- and four-cell embryos, CAR-1 continued to colocalize with P-granules in the germline precursors, but numerous additional smaller CAR-1 containing particles also were evident throughout the cytoplasm of all cells. These smaller CAR-1 containing particles were not detected after the 50 to 100-cell stage. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00012484 | car-1 | Y18D10A.17 | Caenorhabditis elegans |
