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Expr4320
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Full-length VENUS::FBL-1C localizes to pharyngeal and gonadal basal laminas. Transgenes containing either EGF2C or FIBC were localized to both pharyngeal and gonadal basal laminae. In contrast, VENUS::FBL-1D did not localize to either basal lamina. |
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Expr16366
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We found that IDPA-3, IDPB-3, IDPC-1, FIPR-4, and NSPB-12 are expressed exclusively in association with the pharynx and overlap in their localization with the pharynx cuticle. Briefly, tagged IDPA-3 was enriched in the grinder, overlapping the CFW-stained component and lining of the terminal bulb cuticle. In addition, we observed enrichment of tagged IDPA-3 in the presumptive ECM that lies between the terminal bulb and the intestinal valve. Tagged IDPB-3 was expressed weakly and localized exclusively to the pm6 cells and material surrounding the CFW-stained grinder. Tagged IDPC-1 had a similar pattern to that of tagged ABU-14; associating with both the anterior and posterior components of the pharyngeal cuticle. However, tagged ABU-14 appears to localize adjacent to CFW-stained components whereas tagged IDPC-1 overlaps CFW-stained components. Tagged NSPB-12 localized to the anterior pharynx cuticle components exclusively, including that of the buccal cavity, flaps, and anterior channels. Tagged FIPR-4 localized to both anterior and posterior pharynx cuticle components (but not the grinder teeth proper) and the presumptive pharynx-intestinal valve ECM. |
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Picture: N.A. |
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Expr8564
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In double immunostaining experiments with anti-myosin and anti-MIG-6 antisera, MIG-6 appears to be localized near the muscle surface. MIG-6 in larvae also localizes to intestine, pharynx and gonad basement membranes. |
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Expr16370
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We found that IDPA-3, IDPB-3, IDPC-1, FIPR-4, and NSPB-12 are expressed exclusively in association with the pharynx and overlap in their localization with the pharynx cuticle. Briefly, tagged IDPA-3 was enriched in the grinder, overlapping the CFW-stained component and lining of the terminal bulb cuticle. In addition, we observed enrichment of tagged IDPA-3 in the presumptive ECM that lies between the terminal bulb and the intestinal valve. Tagged IDPB-3 was expressed weakly and localized exclusively to the pm6 cells and material surrounding the CFW-stained grinder. Tagged IDPC-1 had a similar pattern to that of tagged ABU-14; associating with both the anterior and posterior components of the pharyngeal cuticle. However, tagged ABU-14 appears to localize adjacent to CFW-stained components whereas tagged IDPC-1 overlaps CFW-stained components. Tagged NSPB-12 localized to the anterior pharynx cuticle components exclusively, including that of the buccal cavity, flaps, and anterior channels. Tagged FIPR-4 localized to both anterior and posterior pharynx cuticle components (but not the grinder teeth proper) and the presumptive pharynx-intestinal valve ECM. |
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Picture: Fig. 3J, 3K. |
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Expr8210
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The pattern of anti-SPON-1 staining in overexpressing animals was similar to that of SPON-1::GFP, including expression in embryonic and larval muscles, the excretory canal, pharyngeal basement membranes and coelomocytes. |
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Expr860
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CLE-1 strongly accumulates in a punctate pattern associated with the nerve ring and the dorsal and ventral nerve cords. This pattern is first visible at the two-fold stage of embryogenesis (~460 min) and persists in all subsequent stages. Beginning in the L4 larval stage, CLE-1 is also observed in basement membranes surrounding the gonad, pharynx, and intestine, and under body wall muscles where it preferentially accumulates at junctions between muscle cells and along dense body lines. The apparently more limited distribution of CLE-1 in early development may result from low sensitivity of detection with the antibody. |
Under body wall muscles where it preferentially accumulates at junctions between muscle cells and along dense body lines |
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Expr3156
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Both reporters expressed GFP in head muscle cells, anterior intestinal cells, posterior intestinal cells, and tail muscle cells. The GFP::FBL-1 fusion protein accumulated around the pharynx, apparently in its basal lamina, and in the intestinal cytoplasm. The pharyngeal accumulation was not dependent on gon-1 activity because GFP::FBL-1 fusion protein was detected around the pharynx in gon-1(0) mutants. |
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Both fbl-1C::Venus and fbl-1D::Venus constructs rescued fbl-1(tk45) mutants, suggesting that Venus fusion proteins are functional. |
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Expr3211
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FBL-1C-Venus was expressed at the anterior and posterior ends of intestinal cells and localized to the gonadal and pharyngeal basement membranes. FBL-1D-Venus was strongly expressed in some of the body wall muscle cells in the head and tail. However, the FBL-1D-Venus was not detected in basement membranes. |
FBL-1C-Venus was expressed at the gonadal and pharyngeal basement membranes. FBL-1D-Venus was was not detected in basement membranes. |
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Colocalization Assay: In order to compare directly the distribution of lam-3 and epi-1, both subunits were co-stained using species-specific secondary antibody conjugates. As the germ layers develop, both subunits are deposited between the layers. However, the staining for laminin A is most intense around the pharyngeal and intestinal precursor cells, whereas staining for laminin B is most intense around the myoblast cells and along epidermal cells. By the onset of elongation, distinct layers of laminin A and laminin B staining can be distinguished, particularly anteriorly between the developing pharynx and the body wall. This indicates that the segregation of the laminin isoforms begins early, before or as organogenesis proceeds. In mnDf90, the two subunits remain differentially localized after elongation. lam-3 is localized to the pharyngeal basement membrane, whereas epi-1 is associated mainly with the body wall basement membranes and only weakly with the pharyngeal basement membrane. These results indicate that each laminin alpha subunit is segregated in the embryo to different adjacent basement membranes and that each membrane retains its unique subunit composition. |
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Expr2622
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The antisera indicate that early expression occurs during gastrulation. epi-1 accumulates between the primary tissue layers near the completion of gastrulation (~250 minutes). epi-1 antiserum stains all the major basement membranes during the remainder of embryogenesis and throughout larval development and in the adult. Although epi-1 staining is weak in the basement membranes surrounding pharynx, intestine, body wall muscle and epidermis, the staining is strong in the basement membranes surrounding gonad, distal tip cell, vulval muscle, intestinal muscle, anal depressor muscle and coelomocytes. These basement membranes are notable in that they are thick and appear to have mainly the alphaB-containing laminin isoform. |
Although the staining is diffuse within most basement membranes, at the muscle/epidermis membrane a distinct pattern is observed. On the muscle surface, a grid-like network that comprises regularly spaced bands running circumferentially and longitudinally is observed. The longitudinal bands correspond to the thin-filament-containing (I-band) region of the muscle myofilament lattice. epi-1 is also strongly detected at muscle-muscle cell boundaries. |
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Expr1028
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Larvae stained with anti-nidogen antiserum show that nidogen is localized to body wall basement membranes. Intense staining is associated with the basement membrane of the body wall muscle. |
basement membrane |
