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mhIs9 rescued the lin-17 T and B cell polarity defects. |
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Expr4251
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mhIs9 males contain a lin-17::gfp construct that was expressed in the membranes of the T, B cells and their descendants as well as the F, P11, P12 and vuval precursor cells. |
membrane |
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Expr1921
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REF-2 was first detected weakly in all 12 P cells just before P-cell migration. REF-2 was present in all P cells as migration occurred and remained in both P cell daughters after division in the ventral cord. This is also the point at which anti-REF-2 staining was strongest. REF-2 then disappears in the Pn.a cell lineage. REF-2 also disappears from the Pn.p cells, although it does so at different rates in different Pn.p cells. REF-2 is present for the longest time in the six unfused Pn.p cells P(3-8).p. REF-2 is also present in P1.p and P2.p shortly after those cells fuse with hyp7, although REF-2 decreases to an undetectable level soon after. REF-2 disappears most rapidly in P(9-11).p, with REF-2 being detectable in only some worms around the time of Pn.p cell fusion. In summary, REF-2 protein levels decrease around the time of Pn.p cell fusion, although they do so less quickly in the cells that remain unfused. REF-2 protein was also detected in the nuclei of the B and Y cells in the tail region during L1. |
nuclei |
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Expr1516
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About 8 hour after hatching, P cells descend into the ventral nerve cord and interdigitate to form a single row of cells of P1-P12. In both sexes, mab-5 was detected in P7-P10. In older larva, mab-5 was detected in P7-P12, but not more anterior cells. In hermaphrodites, expression pattern was similar to males with two exceptions. The p(9-11).app cells and p(9-11).p cells no longer express MAB-5 by L2. In male, descendants of P10 consistently express high levels of MAB-5, those of P9 and P11 express low levels, and those of P7 and P8 express barely detectable levels. P12 descendants express MAB-5 only until the division of P12.a. The descendants of P cells express comparable levels of MAB-5 with exceptions to Pn.p cells, they express lower levels than their neighbors. Expression levels in P descendants were similar to those seen in the neighboring juvenile motoneurons. Both sets of cells expressed MAB-5 in a graded pattern, tapering off toward the anterior. In newly hatched larva, in both sexes, mab-5 was detected consistently in nuclei of P9/10 and P11/12, but not more anterior P cells. mab-5 was also detected at high levels in the posterior juvenile motoneurons, located ventrally. MAB-5 is localized to the posterior of the body, including P7-P12 in the P lineage but not in more anterior cells. Within P7-P12, MAB-5 expression was graded with the highest levels in the P10 descendants. The descendants of any one P cell expressed comparable levels of MAB-5. |
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Also cited by Alper and Kenyon, 2002, Development: 'lin-39 is expressed in P(3-8).p in both sexes' |
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Expr2729
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In embryos, larvaes and adults, expression was observed in the central body region. |
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