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Expr4591
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The full-length dyf-5::gfp construct showed weak GFP expression in many neurons in the head, including amphid and labial sensory neurons and three pairs of neurons in the tail, including the phasmid sensory neurons. In addition, expression was observed in many cells in the male tail. |
This DYF-5::GFP fusion could be detected uniformly in axons, cell bodies, and dendrites. In addition, authors observed strong fluorescence at the transition zones, which connect the cilia with the dendrites, and weak fluorescence uniformly in the cilia. |
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Expr4592
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The full-length dyf-5::gfp construct (see Expr4591) showed weak GFP expression in many neurons in the head, including amphid and labial sensory neurons and three pairs of neurons in the tail, including the phasmid sensory neurons. In addition, expression was observed in many cells in the male tail. The dyf-5ex4::gfp fusion construct essentially showed the same dyf-5 expression pattern, albeit stronger and more restricted to the cell bodies. In addition, DYF-5ex4::GFP could be detected in the CAN cells, neurons associated with the excretory canal and in a pair of neurons in the posterior lateral ganglion. |
This DYF-5::GFP fusion could be detected uniformly in axons, cell bodies, and dendrites. In addition, authors observed strong fluorescence at the transition zones, which connect the cilia with the dendrites, and weak fluorescence uniformly in the cilia. |
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Expr10991
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elt-1 reporter gene expression was first seen around the 20-30 cell stage and was maintained during subsequent early embryogenesis in cells corresponding to hypodermal precursor cells. Just prior to morphogenesis, reporter gene expression could be seen in all the major hypodermal cell nuclei. During formation of the comma stage, embryo reporter gene expression declined in the dorsal and ventral hypodermis but remained high in the lateral seam cells. The seam-cell expression of the reporter gene continued throughout morphogenesis to the pretzel stage although at lower levels. In addition, at the threefold stage of development high levels of expression were seen in a group of cells adjacent to the pharynx in a position consistent with neuronal cells of the retrovesicular ganglion. During postembryonic development relatively low levels of expression were maintained in seam cells from the L1 to the adult stage as were high levels of ELT-1 in neuronal cells of the retrovesicular ganglion. ELT-1 expression was also clearly apparent in several different groups of neuronal cell bodies and axons; those of the ventral cord were the most prominent. Expression was also seen in several neurons in which the axon extended from the ventral cord and encircled the pharynx just in front of the posterior bulb.The reporter gene also showed postembryonic expression in sex-specific structures. In hermaphrodites, ELT-1 expression was seen in the vulval muscles (vm1 and vm2) and in males, in a subset of the lateral seam, the SET cells, that give rise to the sensory rays. |
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Lineage expression: Rn descandents. |
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Expr2930
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Among epidermal cells in the tail, efn-4::gfp was expressed in Rn.a descendants but not in other tail epidermal cells. Expression was first detected in Rn.aa and Rn.ap cells, and expression increased in strength in their descendants. Expression levels among descendants of the same Rn.a cell were similar. Expression was strongest and most frequent in descendants of R2.a and R6.a, intermediate in strength and frequency in R4.a, R5.a, and R7.a, and absent from R1.a and R3.a; expression was not scored in R8.a and R9.a descendants because it was weak and highly variable between individuals. |
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