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Expr4594
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Analysis of a reporter transgene revealed that act-5 expression is restricted to a small subset of cells within the C. elegans alimentary tract and excretory systems. GFP fluorescence was easily detected within a subset of embryonic, larval, and adult cells in transgenic animals, consistent with previous findings on the act-5 expression pattern. Within larvae and adults, GFP expression occurred within the 20 cells that comprise the adult intestine, and in two sets of three associated cells: one just anterior and one posterior to the intestine. The anterior set corresponds to the middle of three sets of pharyngeal-intestinal valve cells (vpi2), whereas the posterior group of associated cells comprises the rectal epithelial cell (rectD, rect_VL, rect_VR). Finally, the single excretory canal cell, which extends processes along the entire length of the worm, also showed GFP expression. |
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Expr11809
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Robust GFP expression of pnc-1a::exon1a::GFP was detected in head and pharyngeal muscle, the two ASK neurons, the two distal tip cells and the three rectal gland cells (RectD, RectVL and RectVR). Whereas the four uv1 cells did not express pnc-1a::exon1a::GFP, four adjacent uv2 cells had moderate levels of GFP expression. Low levels of pnc-1a::exon1a::GFP expression were also detected in the vulval muscle. The pnc-1a reporter overlaps in expression pattern with the pnc-1b reporter only in head muscle and perhaps vulval muscle. |
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Expr9959
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PDF-2::GFP signals could be observed throughout post-embryonic life. The PDF-2::GFP-expressing cells were identified as the interneurons BDUL/R, AVG, AIML/R, RIS, AVD and PVT, the chemosensory neuron pairs PHA and PHB, the motor neurons RID and RIML/R, the sensory neurons AQR and PQR, and less frequently in the PVPL/R interneurons. Outside the nervous system, strong expression was observed in rectal gland cells rectD and rectVL/R, the intestino-rectal valve cells virL/R and three posterior arcade cells in the head. Weak GFP fluorescence could also be observed in four additional head neurons that could not be unequivocally identified. GFP fluorescence was visible in neuronal cell bodies, axons and dendrites. |
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Because of the lack of nuclear or cytoplasmic expression, it was difficult to determine which cells were expressing egl-26::gfp in the animals carrying the translational fusion. Because pWH15 retained egl-26 activity as assayed by ability to rescue ku211 and ku228 mutants when expressed from an extrachromosomal array (2/4 lines rescued), it is assumed that the EGL-26::GFP expression pattern would accurately represent global and subcellular localization of EGL-26. |
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Expr1806
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Consistent with the results from the transcriptional fusion, the expression observed near the top of the vulval lumen in animals carrying the translational fusion corresponds most closely to the position of vulE. The 3-D expression pattern of the EGL-26::GFP translational fusion protein showed expression in a ring around the ventral region of the vulva, in a thicker region near the center of the vulva, and at the apex of the vulva corresponding to where the utse lies separating the vulval and uterine lumens. The thick region of expression in the center of the vulva closely corresponds to the vulE cells. The ring around the ventral region of the vulval most likely corresponds to expression by vulB2 as assayed by the transcriptional fusion. In animals that carry this fusion construct on an extrachromosomal array, kuEx90, expression was observed in many regions of the animal, although not ubiquitously. Expression is strong around the cells of the spermatheca, around the mouth, and lining the pharynx, the rectum, and the excretory canals. Expression was also seen in the pharyngeal intestinal junction cells, transiently during L3 in the anchor cell, in rectal epithelial cells D, VL, and VR, in B and in Y, and in several cells with a neuronal appearance. |
Expression is obvious near the vulva and the uterus only during L4. However, it is often very difficult to tell which cell is actually expressing egl-26::gfp because the fusion protein appears to line the lumen of the uterus and portions of the vulval lumen and is not obviously associated with any particular cell cytoplasm. Even in cases where a cell cytoplasm obviously contains egl-26::gfp, expression is often brighter around the apical edge of the cell. |
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Expr3991
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Expression started at the end of gastrulation. At the 2-fold stage, about 60 cells in the head express ast-1, most of them neurons, but also a few cells in the pharynx. A single cell in the retrovesicular ganglion expresses AST-1::YFP throughout development from the 1.5-fold stage onward. Using an odr-2::CFP marker and checking for overlap in expression, this cell was identified as the AVG neuron. The ALN neurons in the tail weakly express AST-1::YFP in late embryo and early larval stages. Occasionally, a second pair of cells in the tail was seen based on cell body position most likely the rectV cells. Expression in the tail generally is weak and somewhat variable from animal to animal. In the L1 stage, AST-1::YFP coexpressed with glr-1::CFP in AVA, AVD (weak ast-1), AVE (weak ast-1) RMDD and SMDD as well as AVG. AST-1::YFP expression decreases during larval development and is restricted to few neurons, which continue to express throughout the entire life cycle. |
The YFP signal was initially seen only in the nucleus consistent with AST-1 being a transcription factor. Already beginning in the 3-fold stage, a redistribution of the YFP signal was seen until most of the signal is outside the nucleus mainly in spots in the cell body but also in the neuronal processes. |
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Expr9958
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PDF-1::GFP signals could be observed throughout post-embryonic life. pdf-1 reporter expression was found consistently in the interneurons ADAL/R and PVT, the chemosensory neuron pairs ASI, ASK, PHA and PHB, the RMED, RMEV and RID motor neurons, the sensory neuron pair ADE, the PQR mechanosensory neuron and less frequently in the PVPL/R interneurons. Strong non-neuronal expression could be detected in rectal gland cells rectD and rectVL/R, and the intestino-rectal valve cells virL/R. Faint GFP fluorescence appeared to be present in a small number of unidentified head neurons. GFP fluorescence was visible in neuronal cell bodies, axons and dendrites. |
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Picture: Figure S7, Movie 1. |
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Expr8105
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Expression patterns of ipla-1 in the adult stage. ipla-1 is expressed in several unidentified cells in pharynx (probably gland cells of pharynx), pharyngeal-intestinal valve, spermatheca, hypodermal syncytium, seam cells, rectal grand cells and intestinal-rectal valve. Expression patterns of ipla-1 in the larval stages. ipla-1 is expressed in intestine, three rectal gland cells, hypodermal syncytium and seam cells in the L1, L2 and L3 larva. In the L1 stage, ipla-1 expression is also observed in several unidentified cells in the tail. |
While IPLA-1 localizes to cytosol in intestine, spermatheca and gland cells, IPLA-1 localizes to the nuclei of hypodermal syncytium and seam cells. |
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Transgenic Marker: rol-6(su1006). |
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Expr517
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At approximately 260 min after the first cleavage, reporter gene expression is now also detected in nuclei on the dorsal surface of the embryo, corresponding to the position of the major hypodermal cells, and in six nuclei on each ventrolateral aspect of the embryo, corresponding to the position of the ventral hypodermal cells (P cells). Expression was first seen in granddaughters of C.paa and C.aaa about 240 min. after first cleavage. About 260 min. after first cleavage, expression was seen in dorsal and ventral hypodermal cells. Between comma-stage and 3-fold stage, expression was seen in dorsal and ventral hypodermal cells, and in tail and head hypodermis. After the 3-fold stage, expression becomes reduced, but is maintained a low levels in larvae and adults. After the 3-fold stage through adulthood, a digestive-tract component is expressed: probably vpi3D and vpi3V in the pharyngeal intestinal valve, virL and virR in intestinal rectal valve, and rect D, rect VL, rect VR. |
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Reporter gene fusion type not specified. |
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Expr2725
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Expressed in rectal epithelial cells RepD, RepVL and RepVR. Not expressed in intestino-rectal valve, K, K', U, R, B or Y cells. |
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Reporter gene fusion type not specified. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr633
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Two different grd-1 constructs, an amino-terminal grd-1M::gfp and a grd-1C::gfp-lacZ reporter construct are expressed in the rectal cells of larvae to adult, possibly rectal epithelial cells. grd-1M::gfp is expressed the rectal epithelium cells rect D, rect VL, and rect VR. |
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