WormMine: LifeStage WBls:0000020

• Definition: The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.
• Primary Identifier: WBls:0000020
• Public Name: 3-fold embryo Ce

551 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Pharyngeal sensory-motorneurons	m3L	lineage name: ABaraapappp	WBbt:0004471
Pharyngeal sensory-motorneurons	m3R	lineage name: ABaraappppp	WBbt:0004469
Ring interneuron	URXL	lineage name: ABplaaaaappp	WBbt:0004928
Ring interneuron	URXR	lineage name: ABarpapaappp	WBbt:0004926
Neuron class of one interneuron, projects along ventral cord to ring.	PVT	lineage name: ABplpappppa	WBbt:0004070
gland cell of the secretory-excretory system, sends processes to ring, opens into excretory duct.	excretory gland cell	exc gl	WBbt:0005776
Amphid interneuron	AIYL	lineage name: ABplpapaaap	WBbt:0003963
Amphid interneuron	AIYR	lineage name: ABprpapaaap	WBbt:0003961
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
Neuron class of one pharyngeal motorneuron.	M5 neuron	lineage name: MSpaaapap	WBbt:0004465
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASGL	lineage name: ABplaapapap	WBbt:0003892
Neuron class of one pharyngeal interneuron, posterior sensory.	I5 neuron	I5	WBbt:0004740
Neuron, ciliated ending in head, no supporting cells, associated with ILso	BAGL	lineage name: ABalppappap	WBbt:0003824
Neuron, ciliated ending in head, no supporting cells, associated with ILso	BAGR	lineage name: ABarappppap	WBbt:0003823
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASGR	lineage name: ABpraapapap	WBbt:0003891
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASEL	lineage name: ABalppppppaa	WBbt:0003904
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASER	lineage name: ABpraaapppaa	WBbt:0003903
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812
Inner labial neuron	IL1VL	lineage name: ABalppapppaa	WBbt:0004549
Inner labial neuron	IL1VR	lineage name: ABarapppppaa	WBbt:0004547
Inner labial neuron	IL1DL	lineage name: ABalapappaaa	WBbt:0004738
Inner labial neuron	IL1DR	lineage name: ABalappppaaa	WBbt:0004737
Inner labial neuron	IL1L	lineage name: ABalapaappaa	WBbt:0004553
Inner labial neuron	IL1R	lineage name: ABalaappppaa	WBbt:0004551
Amphid socket cell, right	AMsoR	lineage name: ABprpaapapa	WBbt:0003929
Pharyngeal neurosecretory motorneuron, contain serotonin	NSMR	lineage name: ABaraapppaav	WBbt:0004446
Pharyngeal neurosecretory motorneuron, contain serotonin	NSML	lineage name: ABaraapapaav	WBbt:0004457
Amphid neuron, prob. chemosensory	ADFL	lineage name: ABalpppppaa	WBbt:0004003
Amphid neuron, prob. chemosensory	ADFR	lineage name: ABpraaappaa	WBbt:0003999

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	A C. elegans life stage that occurs during the 3-fold embryo life stage		C. elegans life stage occurring during 3-fold embryo	WBls:0000808

13 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts with promoter regions binding DREAM protein complex, according to ChIP-seq of E2F-DP (DPL-1 and EFL-1) and MuvB (LIN-9, LIN-37, LIN-52, and LIN-54) of N2 late embryo.	N.A.	WBPaper00053289:DREAM-target
	Genes that showed significantly increased expression in daf-19(m86);daf-12(sa204) comparing to in daf-12(sa204), at 3-fold embryo stage.	BRB Array Tools were used to identify genes with statistically significant variation in expression. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically differentially expressed in wild-type and mutant populations. Genes with a signal variation of 1.5-fold or greater were selected for subsequent experiments. To reduce false discoveries, a class comparison test was conducted using a multivariate per mutation test with a confidence level of 97% (L1 analysis) and 90% (adults).	WBPaper00053550:daf-19(m86)_upregulated_3-fold-embryo
	Candidate daf-19 up regulated genes with a statistically significant signal variation of 1.5-fold or greater. These were identified using a class comparisons tool from BRB Array Tools.	BRB Array Tools (version 3.3) was subsequently used to identify genes with a statistically significant variation in expression when comparing between the two classes defined in this study. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically different. Genes with a signal variation of 1.5-fold or greater were selectively identified for use in all subsequent experiments. To reduce the chances of false discoveries, a class comparison test was conducted using a multivariate permutation test with the confidence level of 97%.	WBPaper00039866:ClassComp_daf-19_upregulated
	Transcription factors down regulated by unc-120(st364).	N.A.	WBPaper00041222:unc-120(st364)_downregulated
	Transcription factors down regulated by hlh-1(cc561).	N.A.	WBPaper00041222:hlh-1(cc561)_downregulated
	Candidate daf-19 down regulated genes with a statistically significant signal variation of 1.5-fold or greater. These were identified using a class comparisons tool from BRB Array Tools.	BRB Array Tools (version 3.3) was subsequently used to identify genes with a statistically significant variation in expression when comparing between the two classes defined in this study. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically different. Genes with a signal variation of 1.5-fold or greater were selectively identified for use in all subsequent experiments. To reduce the chances of false discoveries, a class comparison test was conducted using a multivariate permutation test with the confidence level of 97%.	WBPaper00039866:ClassComp_daf-19_downregulated
	Genes that showed significantly decreased expression in daf-19(m86);daf-12(sa204) comparing to in daf-12(sa204), at 3-fold embryo stage.	BRB Array Tools were used to identify genes with statistically significant variation in expression. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically differentially expressed in wild-type and mutant populations. Genes with a signal variation of 1.5-fold or greater were selected for subsequent experiments. To reduce false discoveries, a class comparison test was conducted using a multivariate per mutation test with a confidence level of 97% (L1 analysis) and 90% (adults).	WBPaper00053550:daf-19(m86)_downregulated_3-fold-embryo
	Candidate daf-19 down regulated genes with a statistically significant signal variation of 1.5-fold or greater. These were identified using a Significance Analysis of Microarrays (SAM).	Lists of genes were generated using SAM (version 2.2) with a false discovery rate (FDR) of less than or equal to 5% (Q-value <= 5%). Twenty-seven repeated runs of SAM were performed using variable random seed numbers for each run. During each run of SAM, 100 permutations were performed. Genes (n =129 downregulated, n = 1 upregulated) appearing in at least 80% of all twenty-seven runs of SAM were further considered for signal variation filtering, which was used to selectively identify genes with a 1.5-fold or greater variation between the two genetic conditions used for comparison.	WBPaper00039866:SAM_daf-19_downregulated
	Candidate daf-19 up regulated genes with a statistically significant signal variation of 1.5-fold or greater. These were identified using a Significance Analysis of Microarrays (SAM).	Lists of genes were generated using SAM (version 2.2) with a false discovery rate (FDR) of less than or equal to 5% (Q-value <= 5%). Twenty-seven repeated runs of SAM were performed using variable random seed numbers for each run. During each run of SAM, 100 permutations were performed. Genes (n =129 downregulated, n = 1 upregulated) appearing in at least 80% of all twenty-seven runs of SAM were further considered for signal variation filtering, which was used to selectively identify genes with a 1.5-fold or greater variation between the two genetic conditions used for comparison.	WBPaper00039866:SAM_daf-19_upregulated
	Transcription factors up regulated by hlh-1(cc561).	N.A.	WBPaper00041222:hlh-1(cc561)_upregulated
	Transcription factors up regulated by unc-120(st364).	N.A.	WBPaper00041222:unc-120(st364)_upregulated
	Genes with significantly decreased expression in aptf-1(gk794) mutant in pretzel-stage comparing to in N2.	Statistical tests used were Wilcoxon Signed Paired Ranks test, Students t-test or Welch test using Origin software. Error bars are SEM.	WBPaper00049336:aptf-1(gk794)_downregulated_3-fold-embryo
	Genes with significantly increased expression in aptf-1(gk794) mutant in pretzel-stage comparing to in N2.	Statistical tests used were Wilcoxon Signed Paired Ranks test, Students t-test or Welch test using Origin software. Error bars are SEM.	WBPaper00049336:aptf-1(gk794)_upregulated_3-fold-embryo

343 Expression Patterns

• Primary Identifier: Expr4735
• Remark: Reporter gene fusion type not specified.
• Pattern: Expression began at the comma stage and continued from the L1 to adult stages in body wall muscles. The tni-1::lacZ animals showed only weak expression in the body wall muscles.

• Primary Identifier: Expr4736
• Remark: Reporter gene fusion type not specified.
• Pattern: Expression began at the comma stage and continued from the L1 to adult stages in body wall muscles. The tni-2::lacZ construct gave strong expression in all the body wall muscles.

• Primary Identifier: Expr4737
• Remark: Reporter gene fusion type not specified.
• Pattern: Expression began at the comma stage and continued from the L1 to adult stages in body wall muscles. The tni-3::lacZ construct was expressed in all the body wall muscles from the L1 to adult stages. In the adult stage, tni-3::lacZ expression in body wall muscles became weaker with time although strong expression persisted in the head and vulval muscles.

• Primary Identifier: Expr4738
• Remark: Reporter gene fusion type not specified.
• Pattern: tni-4::lacZ was expressed only in the pharynx from the 2-fold to adult stages.

• Primary Identifier: Expr4986
• Remark: Picture: Figure 5B.
• Pattern: Consistent with the ubiquitous expression of sqt-3 throughout the life cycle, the R584 antiserum intensely stains the hypodermal cells and cuticles of all stages. Reactive antigens are first detected in wild-type comma-stage embryos within the hypodermal cells. The signal localizes perinuclearly, presumably in association with the secretory pathway. At the threefold stage, coincident with cuticle secretion, the signal becomes progressively extracellular: by the late pretzel stage, all antigen is detected in alignment with the annular ridges of the embryonic cuticle.

• Primary Identifier: Expr4954
• Remark: Picture: Fig. 2.
• Pattern: In larvae and adults, the circumferential filamentous pattern did not persist, but expression was seen in a subset of head and tail socket cells, the vulva, more faintly the uterus, and the rectum.
• Subcellular Localization: GFP expression was observed in the epidermis from the 1.2-fold stage of elongation to the end of embryogenesis. The GFP formed a circumferential filamentous pattern that was spatially and temporally strikingly reminiscent of the circumferential actin microfilament pattern. Indeed, actin staining in a strain expressing the RGA-2::GFP construct showed that the two networks coincide.

• Primary Identifier: Expr4956
• Remark: Picture: Fig. 5, Fig 6.
• Pattern: NAB-1::GFP expression is restricted to epithelia and neurons. The earliest expression was observed in the hypodermis of 2-fold-stage early embryos. Immediately prior to hatching, this expression became restricted to the epithelial excretory canal and the nervous system, including the central nervous system and the motoneurons (dorsal and ventral nerve cords. In L3 and L4 larvae, NAB-1::GFP also localized transiently at the membranes of the developing vulva epithelia. Also expressed in distal tip cell (pers. comm. from Wesley Hung 11-17-07.)
• Subcellular Localization: NAB-1::GFP puncta partially co-localized with the synaptic-vesicle protein SNT-1 and the active-zone protein UNC-10, suggesting that NAB-1 is present in presynaptic regions that are associated with vesicle pools and active zones. Similar to NAB-1, SAD-1 also showed co-localization with SNT-1. NAB-1::GFP and SAD-1 also showed partial co-localization, where each NAB-1::GFP punctum was associated with SAD-1 staining.

• Primary Identifier: Expr4940
• Pattern: strong bwm, vul, mu int, occaisonal weak pharyngeal, spermatheca, faint VNC, head neurons; embryonic bwm starts at 3-fold and very strong in L1s.

• Primary Identifier: Expr4941
• Pattern: strong bwm in 3-fold embryos; no other muscle seen in larvae or adults; saw faint bwm in 1.5 fold embryo but no earlier.

• Primary Identifier: Expr4942
• Pattern: strong bwm, vul, anal dep, mu int; strain A shows embryonic bwm starting at 1.5 fold and strong at 3-fold

• Primary Identifier: Expr4943
• Pattern: strong 3-fold emb bwm that fades and becomes mosaic in late L's and adults, vul, anal dep, spinchter, mu int; seam cells in one line at least

• Primary Identifier: Expr4944
• Pattern: strong bwm, vulval muscle and anal depressor, strong bwm at 3-fold into L1s, earliest exp is faint bwm in 2-fold seen in line B.

• Primary Identifier: Expr4931
• Pattern: strong bwm, pharyngeal, vulval, and anal depressor; a bit mosaic; starts at 3-fold?

• Primary Identifier: Expr4917
• Pattern: Moderate bwm, mosaic, tail hyps, earlies bwm is comma with 1-2 cells +, by 3-fold fairly strong in many bwm; early gut at 12E, strong gut at 1.5 fold then fades to cytoplasmic and gone.

• Primary Identifier: Expr4919
• Pattern: Strong pharyngeal, vulval, bwm, anal dep, mu int; 3-fold earliest embryonic.

• Primary Identifier: Expr4393
• Pattern: The GFP signal was first detected from the 3-fold stage of embryogenesis during development. At the 3-fold stage, GFP expression was observed in hypodermal and intestinal tissues. During larval and adult stages, GFP was strongly detected in the hypodermis and intestine as well as the excretory cells.

• Primary Identifier: Expr4344
• Pattern: qua-1pro::GFP was found to be expressed in the hypodermal cells covering the whole body from the tip of the nose to the tip of the tail spike, but not in the lateral hypodermal cells, i.e., the seam cells. Furthermore, expression was seen in the excretory duct and pore cells from threefold stage embryos to adults. However, in adults the GFP intensity appears weaker than in larvae. In L1 larvae, qua-1 is expressed in two, sometimes four, cells of the anterior as well as the posterior of the intestine and a rectal epithelial cell. In addition, transient expression was observed in the P cells in L1 in the ventral side of the animal and in a few sensilla support cells in the head. In adults, qua-1pro::GFP is transiently expressed in a few cells in the head that remain to be identified.

• Primary Identifier: Expr4264
• Pattern: Expressed in body wall muscle cells, pharyngeal muscles, rectal gland cells, vulval and uterine muscles, and a subset of neurons in the head and ventral nerve cord. The expression was first detected in the embryo at the 1.5-fold stage and continued to be expressed until adulthood. In this stage, cells with position corresponding to P cells showed also GFP expression. In order to determine if the expression of GFP was in epidermal lineages, including P cells and their descendants, authors prepared double transgenic lines expressing GFP from promoter 1 of nhr-40 on the background of SU93 line that expresses an AJM::GFP membrane marker. This confirmed the expression of nhr-40::gfp in epidermal precursors P cells and their neuronal progeny within the ventral neuronal cord. However, the GFP was not observed in ventral epidermal cells that originated from P cells. The nhr-40::gfp was not observed in seam cells that are also marked by an AJM::GFP. The muscle pattern of expression was partially lost with truncations of this promoter fragments (-1013 and -682 bp); however, other aspects of expression were retained.

• Primary Identifier: Expr4266
• Pattern: Reporter genes that begin at -3190, -2021, -1248, and -517 bp upstream of the ATG of this alternate first exon 1 were expressed in body wall muscle cells, neurons in the head, nerve ring, ventral and dorsal nerve cords, neurons, and some epidermal cells in the tail. Weaker expression was also observed in pharyngeal muscles. The expression from promoter 2 started in the embryos at the 1.5-fold stage and was continuous throughout development. Expression from this internal promoter element is observed in vulval cells at the L4 stage and in adult hermaphrodites, including the uterine vulval cells uv1, 2, 3, and surrounding epithelium.

• Primary Identifier: Expr4608
• Pattern: Clear expression was shown in two of the hypodermal syncytia, hyp4, which surrounds the anterior part of the pharynx, and hyp7, which surrounds the mid-body region of the animal. The earliest detectable GFP expression can be observed in comma stage embryos, where there is abundant expression in the newly generated hyp4 nuclei and, albeit less frequently, in hyp7 nuclei. This expression pattern remains consistent throughout embryonic and larval development up until adulthood. There is faint expression in the hindgut, which most likely represents background expression of the reporter.

• Primary Identifier: Expr4466
• Pattern: Exclusively expressed in the pharyngeal muscle or marginal cells from the embryonic comma stage to adults.

• Primary Identifier: Expr4559
• Pattern: In the adult, unc-27gfp (bxEx97), a rescuing reporter, is expressed exclusively in body-wall muscles, inner and outer longitudinal muscles, anal depressor, sphincter, and male-specific diagonal muscles. GFP expression is first detected in the 3-fold embryo in embryonic body-wall muscles and is maintained after hatching into the adult stage.

• Primary Identifier: Expr4517
• Pattern: ife-2 is highly expressed in all somatic tissues of adult animals, including the pharynx, the intestine, body wall muscles, the hypodermis, neurons and the canal cell. Expression is high during all post-embryonic developmental stages, throughout adulthood, and is detectable in 2-fold stage embryos.

• Primary Identifier: Expr7745
• Remark: Clone: pUL#JRH/AF08
• Pattern: Comma stage embryos show low levels of expression in virtually all cells but higher levels in outer cells. Late stage embryos show more localized expression in cells down sides and in the head. Post-embryonically expression is seen in several cells in the head, in the excretory cell, in cells of the developing and adult vulva, in rectal epithelia, & in seam cells.

• Primary Identifier: Expr12443
• Pattern: A GFP fusion gene with 7.7 kb of nsy-4 upstream sequence was expressed beginning at the comma stage of embryogenesis and continuing until the adult. At the 2-fold stage of embryogenesis, expression was prominent in the excretory cell and in anterior epidermal cells. At the first larval stage, the nsy-4 reporter transgene was expressed in the excretory cell, in epidermal cells in the head (some or all of hyp 1-6) and tail (some or all of hyp 8-11), and in the P neuro-epidermoblasts, both before and after their ventral migration. Weak nsy-4::GFP expression was detectable in a few neurons. In three appropriate mosaic animals identified at the L1/L2 stage, nsy-4::GFP was observed in the AWC cell body. Expression was not detectable in AWC in older animals.

• Primary Identifier: Expr9163
• Remark: Picture: Fig. 3A, 3B.
• Pattern: GFP was expressed in nuclei of the hypodermal cells and in some intestinal cells. The GFP signal was detected from 3-fold stage embryos to young adult, and was exactly co-localized with EMR-1::RFP which was expressed under the control of the rol-6 promoter.

• Primary Identifier: Expr3720
• Pattern: In C. elegans males, nph-4::GFP expression in the head was seen in the amphid neurons, the outer labial neurons, and a cell group that could be compatible with the male-specific CEM neurons. In the male tail, nph-4::GFP expression was seen in the lumbar and cloacal ganglia. nph-4 expression in hermaphrodites was found in ciliated amphid neurons, the outer labial neurons, and the phasmid neurons in the tail. Developmental regulation of expression for nph-4::GFP was found to be similar to nph-1, appearing at the 1.5-fold stage and lasting through adulthood.

• Primary Identifier: Expr9039
• Remark: Picture: Fig 5G, 5H, 5I.
• Pattern: The expression of nhr-206 GFP reporter transgenes starts during the comma stage of embryogenesis, and is initially seen in four unidentified cells localized in the head region. By the 2-fold stage, embryonic expression is observed in the pharynx with weaker expression in intestine. This expression pattern continues throughout all larval stages and in adults with pronounced anterior pharyngeal expression. The reporter genes were also strongly expressed in rectal gland cells, the anal sphincter, and in epidermal cells in the tail. Weaker expression was also observed in the vulva and spermatheca. In males, expression was visible in male specific neurons of the tail and rays.

• Primary Identifier: Expr9040
• Remark: Picture: Fig 5J, 5K, 5L.
• Pattern: The expression of nhr-208 GFP reporters started in embryos at the 1.5-fold stage within the pharynx, intestinal sphincter, and epidermal cells in the tail. By the 3-fold stage of embryogenesis, additional expression was observed in rectal gland and surrounding cells. During all larval stages, strong expression of the transgenes was visible in pharyngeal and unidentified head neurons, the pharyngeal-intestinal valve cell, the posterior part of the intestine, the intestinal sphincter, two rectal gland cells, the intestinal-rectal valve cell, and the epidermal hyp10 cell. In males, the expression was seen in several rays (6-8) and other male specific neurons.

• Primary Identifier: Expr9041
• Remark: Picture: Fig 5M, 5N, 5O, 5P.
• Pattern: The expression of nhr-207 GFP reporters began in 1.5-fold embryos in pharyngeal and epidermal cells. In 3-fold stage embryos, expression was observed in pharyngeal neurons, intestinal cells, the intestinal-rectal valve, and the sphincter. This pattern of expression was present during all larval stages and in adults. In larvae and in adults, additional expression was observed in the pharyngeal-intestinal valve and spermathecae. In males, the expression was seen in male specific neurons, including rays.

0 Followed By

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019

32 Sub Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The time period encompassing the 523rd minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		523 min post first-cleavage Ce	WBls:0000634
	The time period encompassing the 522nd minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		522 min post first-cleavage Ce	WBls:0000633
	The time period encompassing the 521st minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		521 min post first-cleavage Ce	WBls:0000632
	The time period encompassing the 527th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		527 min post first-cleavage Ce	WBls:0000638
	The time period encompassing the 526th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		526 min post first-cleavage Ce	WBls:0000637
	The time period encompassing the 525th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		525 min post first-cleavage Ce	WBls:0000636
	The time period encompassing the 524th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		524 min post first-cleavage Ce	WBls:0000635
	The time period encompassing the 531st minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		531 min post first-cleavage Ce	WBls:0000642
	The time period encompassing the 530th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		530 min post first-cleavage Ce	WBls:0000641
	The time period encompassing the 529th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		529 min post first-cleavage Ce	WBls:0000640
	The time period encompassing the 528th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		528 min post first-cleavage Ce	WBls:0000639
	The time period encompassing the 535th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		535 min post first-cleavage Ce	WBls:0000646
	The time period encompassing the 534th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		534 min post first-cleavage Ce	WBls:0000645
	The time period encompassing the 533rd minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		533 min post first-cleavage Ce	WBls:0000644
	The time period encompassing the 532nd minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		532 min post first-cleavage Ce	WBls:0000643
	The time period encompassing the 539th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		539 min post first-cleavage Ce	WBls:0000650
	The time period encompassing the 538th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		538 min post first-cleavage Ce	WBls:0000649
	The time period encompassing the 537th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		537 min post first-cleavage Ce	WBls:0000648
	The time period encompassing the 536th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		536 min post first-cleavage Ce	WBls:0000647
	The time period encompassing the 543rd minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		543 min post first-cleavage Ce	WBls:0000654
	The time period encompassing the 542nd minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		542 min post first-cleavage Ce	WBls:0000653
	The time period encompassing the 541st minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		541 min post first-cleavage Ce	WBls:0000652
	The time period encompassing the 540th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		540 min post first-cleavage Ce	WBls:0000651
	The time period encompassing the 547th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		547 min post first-cleavage Ce	WBls:0000658
	The time period encompassing the 546th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		546 min post first-cleavage Ce	WBls:0000657
	The time period encompassing the 545th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		545 min post first-cleavage Ce	WBls:0000656
	The time period encompassing the 544th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		544 min post first-cleavage Ce	WBls:0000655
	The time period encompassing the 550th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		550 min post first-cleavage Ce	WBls:0000661
	The time period encompassing the 549th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		549 min post first-cleavage Ce	WBls:0000660
	The time period encompassing the 548th minute post first cleavage at 20 Centigrade with respect to Sulston's lineage tree.		548 min post first-cleavage Ce	WBls:0000659