WormMine: ExpressionPattern Expr13342

• Pattern: PLST-1::GFP localized to both lamentous and punctate structures at the cortex of newly fer- tilized zygotes. PLST-1::GFP continued to be expressed ubiquitously during the first 5 h of embryogenesis, with prominent enrichment at cell-cell contacts, and during elongation, PLST-1::GFP was enriched in the buccal region of the epidermis, pharynx, and intestine. In adult hermaphrodites, PLST-1 expression was found in the socket cells of amphids, pharyngeal- intestinal valve, intestinal lumen, syncytial and cellularized gonad, vulval cells, intestinal-rectal valve, and socket cells of phasmids.The lamentous and punctate structures formed by PLST- 1::GFP in the newly fertilized zygote were also labeled by Lifeact::RFP, an F-actin reporter. The strong colocalization of the PLST-1::GFP and Lifeact::RFP signals, from before polarization, during polarity establishment and maintenance, and throughout cytokinesis, is evident in the representative line scans and from calculated Pearson's correlation coefficients with values >0.7 (n ≥ 8). This suggests that PLST-1::GFP associates with all cortical F-actin.
• Primary Identifier: Expr13342

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00022425	plst-1	Y104H12BR.1	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003