WormMine: ExpressionPattern Expr13175

• Pattern: lgc-46 is broadly expressed in the nervous system, including motor neurons. Co-expression of Plgc-46-GFP with markers for cholinergic (Punc-17-mCherry) and GABAergic (Pttr-39-mCherry) neurons revealed that lgc-46 is strongly expressed in cholinergic motor neurons, and weakly expressed in GABAergic motor neurons.
• Primary Identifier: Expr13175
• Subcellular Localization: In the DA9 cholinergic motor neuron, which synapses onto dorsal body wall muscles, LGC-46::GFP was detected only in the dorsal side of L4 and adult animals. These results indicate that LGC-46 primarily localizes to the axonal compartment of cholinergic motor neurons. To determine if the punctate pattern of LGC-46::GFP corresponded to presynaptic terminals, we co-immunostained LGC-46::GFP together with endogenous active zone protein RIM/UNC-10. LGC-46::GFP displayed a high degree of overlap with UNC-10. Quantitative analyses of colocalization indicate that LGC-46 localizes to the release sites of synaptic vesicles (SVs). Additionally, LGC-46::GFP showed co-localization with a synaptic vesicle marker mCherry::RAB-3 expressed in cholinergic motor neurons. To our knowledge LGC-46 is the first reported LGIC localized to presynaptic terminals in C. elegans.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities.	nervous system		WBbt:0005735
neuron that uses GABA as a vesicular neurotransmitter.	GABAergic neuron		WBbt:0005190
neuron that uses vesicular acetylcholine as a neurotransmitter.	cholinergic neuron	ACh neuron	WBbt:0006840

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00022106	lgc-46	Y71D11A.5	Caenorhabditis elegans

0 Life Stages