WormMine: ExpressionPattern Expr9233

• Pattern: Transgenic embryos harboring a ral-1 promoter-driven gfp fusion construct showed broad embryonic GFP expression. Post-embryonically, GFP was observed in excretory canals, a small number of neurons, and was expressed dynamically in vulval lineages. Prior to EGF induction, GFP was expressed in all VPCs, but at the time of induction, GFP was restricted to P5.p, P6.p and P7.p, cells receiving the EGF signal. Soon thereafter expression was extinguished in the presumptive 1 cell (P6.p), persisted strongly in presumptive 2s (P5.p and P7.p), and was faintly restored in presumptive 3s. Further dynamic expression changes were seen in later vulval development, after fate specification.
• Primary Identifier: Expr9233
• Remark: After EGF induction, GFP was restricted to P5.p, P6.p and P7.p, cells receiving the EGF signal.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775
cell that has the potential to form structural part of a vulva.	vulval precursor cell	VPC	WBbt:0007809

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00021811	ral-1	Y53G8AR.3	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003