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Picture: Fig. 1G, 1H, Fig S1G-L. |
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Marker30
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Expressed in CEPsh glia and in sheath and socket glia of inner and outer labial sensilla, and of the deirid. |
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Expr13568
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ztf-6::gfp expression is first observed in late embryogenesis (threefold stage). In early larval stages, the ztf-6::gfp-expressing cells include head hypodermal cells, head muscle cells, neurons, and ectodermal blast cells along the body (all P and all V cells) and in the tail. Starting in the L2 stage, additional neurons in P cell-derived ventral cord motor neurons express ztf-6::gfp. Because of the postdeirid loss phenotype of ztf-6 mutants, we examined the postdeirid lineage in more detail. We observe ztf-6 expression in the V5 cell of freshly hatched L1 animals. Upon division of the V5 cell into a posterior and anterior daughter, we observe expression in both the anterior and posterior daughters of the V5 cell. The descendent of the posterior V5.p daughter, V5.pa (the founder of the postdeirid lineage), and V5.pp also continue to express ztf-6::gfp. Within the V5.pa lineage, expression of ztf-6::gfp is retained in the blast cells that generate the glial cells and the PDE and PVD neurons (v5.paaa, V5.papa, V5.paap, V5.papp). No expression is detected at later stages in this lineage. |
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The same pattern of expression was also noted when a genomic fragment spanning nucleotides I-1630 was used to drive the GFP reporter. |
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Expr943
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Both hermaphrodites and males expressed GFP in a group of cells around the pharyngeal corpus, which represented either the sheath or socket cells (PDEso) in the tail, as defined by their openings, position and morphology. GFP expression in these sensory support cells was detected soon after their birth and was maintained into adulthood. A GFP signal was detected in all the ray structure cells of the male tail, but not in the hypodermal tissue. Expression in nine pairs of structure cells began just prior to the ray retraction period, peaked during the retraction and declined upon completion of ray extension. |
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