WormMine: ExpressionPattern Expr8634

• Pattern: A different expression pattern of CeNF-Y genes was observed in infertile worms (12 days after L1 stage). The positivity in the gonads disappeared, while CeNF-YB and CeNF-YC expression decreased in the pharingeal secretory cells and the neurons. Apart from the gonads and developing embryos, the CeNF-Y mRNAs were detected in other tissue from C. elegans. In 6-day-old worms, CeNF-YB and CeNF-YC, but not CeNF-YA1 nor CeNF-YA2, were expressed in the cytoplasm of the secretory cells located in the pharyngeal terminal bulb wall, in the neuron cell bodies of the small ganglia surrounding the pharynx and in the neurons running anteriorly to the sensory organs in the animal's head. NF-YC was also found in the excretory cell. Subunits were not expressed in any other organs, such as the intestine, the hypodermis and body wall muscle surrounding the pseudocoelomic space. The in situ hybridization procedure revealed the expression of CeNF-Y gene subunits in adult, fertile, hermaphrodite (6 days after L1 stage) C. elegans. High mRNA expression of all the subunits was evident in various parts of the gonads with differing degrees of intensity. In particular, positivity increased in oocytes from the distal to the proximal arm of the gonad in function of the stage of maturation. The cytoplasm of the syncytial ovaries and immature oocytes at the beginning of the proximal arm of the gonad were weakly positive, while oocytes, which increased in size along the oviduct toward the spermatheca, were strongly positive. Fertilized oocytes present in the uterus were strongly positive. In the late embryo stage, the expression of CeNF-Y mRNAs did not appear to be uniformly distributed, and presented an asymmetrical organization with anterior-posterior polarity.
• Primary Identifier: Expr8634
• Remark: Picture: Fig 2, 3, 4. The controls of the in situ hybridiza tion reactions showed negative results.
• Subcellular Localization: cytoplasm

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812
organ producing either sperm or ova.	gonad		WBbt:0005175

4 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00011614	nfya-1	T08D10.1	Caenorhabditis elegans
WBGene00017742	nfyc-1	F23F1.1	Caenorhabditis elegans
WBGene00021132	nfyb-1	W10D9.4	Caenorhabditis elegans
WBGene00043056	nfya-2	Y53H1A.5	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003