WormMine: ExpressionPattern Expr1864

• Pattern: Expressed in muscle.
• Primary Identifier: Expr1864
• Subcellular Localization: Both UNC-60B and CeTM became detectable in muscle cells at a very early stage of muscle development (~290 min), nearly at the same time. At the comma stage (290350 min), both UNC-60B and CeTM are diffusely localized in the cytoplasm and positions of nuclei are devoid of staining. After the 1.5-fold stage (~430 min), CeTM was gradually localized to a narrow region which represented myo-fibrils as determined by double staining with anti-actin antibody, whereas UNC-60B remained in the diffuse cytoplasm. This differential localization of UNC-60B and CeTM was maintained throughout the later embryonic development. In adult body wall muscle, CeTM and UNC-60B were also localized to different regions of thin filaments. Interestingly, the staining patterns of CeTM and actin were different. CeTM was localized in linear patterns, whereas actin was localized in ladder-like patterns in which the regions of dense bodies were devoid of staining. UNC-60B was stained as dotted lines between two lines of CeTM with little overlapping zones. Double staining of actin and UNC-60B showed that UNC-60B was localized to the central areas of the actin ladders between dense bodies. Double staining of vinculin, as a marker for dense bodies, and UNC-60B revealed that UNC-60B filled the gaps between dense bodies but was not colocalized with them. The shape of the UNC-60Bpositive dots was irregular, suggesting that association of UNC-60B with myofibrils is unstable and dynamic.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Contractile cell that makes up muscle tissue.	muscle cell		WBbt:0003675

2 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002978	lev-11	Y105E8B.1	Caenorhabditis elegans
WBGene00006794	unc-60	C38C3.5	Caenorhabditis elegans

9 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013