WormMine: ExpressionPattern Expr1617

• Pattern: Expression of lin-28:GFP is greatest in late embryos and L1 larvae, detectable but diminished in the L2, and greatly diminished by the L3 and L4 stages. None of the animals that were fluorescing at the L1 did so at the L4 stage. Some neuron cell bodies, particularly those in the head, can occasionally be seen fluorescing weakly in late stages, and fluorescence in hypodermis and muscle is generally undetectable in L3-stage or older animals. In transgenic L1 larvae, fluorescence can be seen in diverse cell types, including hypodermis, muscle, and neurons, but not in the germline. Lineages that are abnormal in lin-28 mutants, such as lateral and ventral hypodermis, express the lin-28:GFP fusion. Other cell types, such as head neurons and body muscle also express lin-28:GFP. The fluorescence is most intense in neurons and occasionally in hypodermal blast cells, and is often weak in or absent from the intestine. Northern and RNase protection analysis reveals that the lin-28 mRNA is present throughout postembryonic development.
• Primary Identifier: Expr1617
• Subcellular Localization: The fluorescence is in the cytoplasm in all of these cells, though occasionally fluorescence can be detected in some nuclei or nucleoli.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
Contractile cell that makes up muscle tissue.	muscle cell		WBbt:0003675

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003014	lin-28	F02E9.2	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002