WormMine: ExpressionPattern Expr57

• Pattern: Antibody staining: nuclear stained blastomeres from at least 2 cell stage on. All blastomeres stain up to about the 200 cell stage. Pattern then becomes dynamic. Dorsal blastomere expression turns off, leaving strong expression in head, ventral cord region, and tail at comma stage. As embryogenesis continues, expression fades in most cells so that by hatching, only ~12 cells positive for expression in head and ~6 cells in tail (all presumptive neurons). Post-embryonic staining unknown. lacZ expression: 1 construct in 3 independant lines shows onset of expression in comma stage embryos in a few presumptive neuronal precursors in the head. In later stage embryos expression is in the head, ventral half and tail of embryos. This expression co-localizes with areas of neurogenesis. Post-embryonic expression in occasional neurons in head, tail, or midsection.
• Primary Identifier: Expr57
• Remark: Sequence Genbank accession numbers cDNA = U13614; genDNA = U30248 Vertebrate Homologs: Human and mouse protein(s) E12/E47. Invertebrate Homologs: Drosophila protein "Daughterless". Vertebrate E12 acts with MyoD. early embryonic expression profile would be consistant with a role of CeE12/Da in dosage comp. analogous to Daughterless in Dros.mel. but not evidence for it. Later embryonic expression suggests CeE12/Da is important for neurogenesis, probably a pairing partner for the acheate-scute homologs in the worm. This would be similar function to its role in vertebrates and Dros.mel.. CeE12 does not appear to be in body wall muscle cells therefore not a pairing partner for CeMyoD; this result is in constrast to vertebrate functions. life_stage summary : postembryonic

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001949	hlh-2	M05B5.5	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003