WormMine: ExpressionPattern Expr1199

• Pattern: In wild-type worms, EGL-8 staining primarily in the nervous system and the intestine. EGL-8 staining observed in most or all neurons, although the amount of staining varied between individual neurons. In general, neurons in the head and tail ganglia showed stronger staining than the motor neurons of the ventral cord. In the head ganglia, one pair of amphid neurons showed especially strong staining. Staining was not detected in the sublateral processes or in the dorsal cord. EGL-8's intestinal staining was restricted to two lines of staining running down the long axis of the body and to pairs of loop-like structures at regular intervals. The strongest staining was in the posterior region of the intestine. Confocal microscopy determined that the two lines of staining are on the luminal (apical) side of the intestinal cells.
• Primary Identifier: Expr1199
• Remark: The absence of this staining in egl-8 (md1971) and egl-8 (n488) demonstrates its specificity. The lines of EGL-8 staining colocalize with the staining of MH27, a monoclonal antibody that stains epithelial and intestinal adherens junctions. MH27 staining also extended part of the way around each of the loops of EGL-8 staining, which may represent sites where the ends of the intestinal cells join together.
• Subcellular Localization: Within neurons, EGL-8 stained in both cell somas and in processes.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001177	egl-8	B0348.4	Caenorhabditis elegans

0 Life Stages