WormMine: ExpressionPattern Expr2879

• Pattern: RFP-1-specific staining is observed in nuclei, but not in nucleoli, of C. elegans embryos. In L1 larvae, specific RFP-1 staining was observed in the nuclei of pharyngeal, intestinal, and nerve ring cells, but not in nucleoli. The nuclear staining of RFP-1 in most tissues was observed in L2, L3, and L4 animals. In adult animals, RFP-1 was localized to the nuclei of oocytes and germ cells in the syncytial gonad. Again, the nucleoli did not stain for RFP-1. Competition with RFP-1 abolished this signal. RFP-1 nuclear staining was also observed in pharyngeal and intestinal cell nuclei in the head region of the adult animal.
• Primary Identifier: Expr2879
• Remark: In a negative control, embryos incubated only with the secondary anti-rabbit antibody showed very weak background staining.
• Subcellular Localization: Expressed in nuclei but not in nucleoli.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
any of 20 large epithelial cells which form a tube and are mostly situated as bilaterally symmetric pairs around the tubular lumen. Each of these cell pairs forms an intestinal ring ( II-IX int rings). The most anterior intestinal ring (int ring I), however, is made of four cells. Intestinal cells contain large nuclei with large nucleoli and numerous autofluorescent granules in their cytoplasm.	intestinal cell		WBbt:0005792

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007008	rfp-1	R05D3.4	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003