WormMine: ExpressionPattern Expr3269

• Pattern: A second GFP fusion construct pPDW06-9, which contains the same promoter for W06B3.2c and the entire genomic region of W06B3.2a/c except for the termination codon, rescued the phenotypes of the sma-5 mutant, and looks to be expressed in the same regions as was pPDW06-1a. Fusion protein is localized throughout the intestine. The third construct pPDW06-c carries the 4.9 kb sequence upstream of exon 1 and a GFP gene. GFP in this promoter fusion is expressed in hypodermis and pharynx. pPDW06-1a expresses a GFP fusion with N-terminal five amino acids of W06B3.2a/c under a control of promoter for W06B3.2c of 1.6 kb. GFP expression was found in intestine and excretory cell, in all stages of a transgenic line carrying extrachromosomal arrays of pPDW06-1a. In the intestine, the four most anterior cells show stronger GFP expression, although entire intestine is fluorescent. The expression in the excretory cell was confirmed by comparison with the expression pattern of vha-1p::gfp that is predominantly expressed in the excretory cell. GFP in the excretory cell is seen continuously along the lateral lines for most of the length of the worm. Weak expression in hypodermis is also seen.
• Primary Identifier: Expr3269

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004859	sma-5	W06B3.2	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003