WormMine: ExpressionPattern Expr9885

• Pattern: We detected puncta of cwn-2p::CWN-2::Venus mostly around the pharynx. We detected these puncta on the hypodermis, including the seam cells, suggesting its diffusion from the pharyngeal region. Although it was also reported that the cwn-2 promoter is active in the intestine, albeit weaker than in the pharynx, we detected cwn-2p::CWN-2::Venus puncta only in the anterior region, along the boundary between the intestine and muscle or hypodermis. These observations indicate that CWN-2 is mostly distributed to the anterior side of the animal.
• Primary Identifier: Expr9885
• Reporter Gene: [cwn-2p::CWN-2::Venus] translational fusion. cwn-2p::CWN-2::Venus was constructed from PCR fragments containing its promoter region (6.1 kb), and the entire coding region was amplified by PCR from the fosmid WRM0622bE06 inserted into a pPD95.75::wVenus derived from pPD95.75 (a gift from A. Fire) and containing the Venus gene optimized for C. elegans codon usage in place of the GFP gene. --precise ends.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000858	cwn-2	W01B6.1	Caenorhabditis elegans

0 Life Stages