WormMine: ExpressionPattern Expr2295

• Pattern: C-terminal construct: Expression begins late in embryogenesis, and continues through larval stages into adulthood. In larvae, expression is strong in the hypodermis, pharynx and intestine. sma-3 expression in the hypodermis is seen throughout the large hypodermal syncytium hyp7, but not in the lateral hypodermal blast cells (the seam cell). Expression of the N-terminal construct is similar, although much weaker, even after integration. Again, the nuclear fluorescence is prominent in the pharynx, intestine and hypodermis.
• Primary Identifier: Expr2295
• Subcellular Localization: C-terminal construct: Nuclear accumulation in all of these tissues is strong. This nuclear localization does not depend on the activity of sma-6, however. When the integrated N-terminal construct array (qcIs6) was crossed into sma-4(e729) or sma-2(e502) mutant backgrounds, the nuclear localization did not change significantly. When the array is crossed into sma-6(wk7) mutants, the protein became evenly distributed between the cytoplasm and the nucleus in many but not all animals. Thus, the nuclear accumulation of SMA-3::GFP is enhanced by but not dependent on activation by the type I receptor. Determining whether this extensive nuclear localization is characteristic of the endogenous SMA-3 protein must await the development of SMA-3 antibodies.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004857	sma-3	R13F6.9	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003