WormMine: ExpressionPattern Expr7877

• Primary Identifier: Expr7877
• Remark: Picture: Figure 2A, Figure S3.
• Subcellular Localization: The fluorescently tagged protein was expressed on the basolateral surface of the intestine, exclusively in the most posterior intestinal cells, consistent with previous observations (see Expr2235 and Expr2242). To confirm basolateral expression, authors exposed the posterior intestine in situ without exposing the apical, luminal surface to acidic media, which quenched the majority of the pHluorin::PBO-4 fluorescence. The expression pattern of PBO-4 demonstrates that the protein resides on the basolateral surface of the intestine.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003943	pbo-4	K09C8.1	Caenorhabditis elegans

0 Life Stages