WormMine: ExpressionPattern Expr11272

• Pattern: Expression of pcni-1::GFP was found in many tissues, including widespread distribution in the nervous system. CNI-1 was expressed in all GLR-1-expressing neurons, including AVA.
• Primary Identifier: Expr11272
• Subcellular Localization: CNI-1 and GLR-1 were in close apposition to SEC-24/COPII puncta, which mark putative ER export sites. CNI-1 was also expressed in the processes of AVA, where it was distributed in a punctate pattern that colocalized with the ER/Golgi marker, PISY-1. The punctate colocalization of GLR-1 with CNI-1 suggests that CNI-1 might also function at synapses and that is expressed on the cell surface. Surface expression of CNI-1 was also supported by in vivo antibody labeling experiments. These results indicate that CNI-1 is not restricted to the cell body or intracellular organelles and that CNI-1 is present at synapses. In neuronal cell bodies, CNI-1::GFP appeared punctate and distinctively clustered at perinuclear sites, suggestive of localization to the ER. By colocalization studies the authors confirmed that CNI-1 is expressed in discrete regions of the ER/Golgi where it colocalized with GLR-1.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities.	nervous system		WBbt:0005735
Ventral cord interneuron, synapses onto VA, DA, and AS motor neurons; formerly called alpha	AVAL	lineage name: ABalppaaapa	WBbt:0003870
Ventral cord interneuron, synapses onto VA, DA, and AS motor neurons; formerly called alpha	AVAR	lineage name: ABalaappapa	WBbt:0003869

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00011648	cni-1	T09E8.3	Caenorhabditis elegans

0 Life Stages