WormMine: ExpressionPattern Expr1584

• Pattern: The large majority of ceh-10 mRNA is found in the embryo and likewise the majority of ceh-10 directed reporter gene expression is also found within the embryo. The earliest point at which ceh-10/lacZ expression can be detected is in a single nucleus at the extreme anterior pole of the embryo, at mid-proliferation stage when the embryo has approximately 350 cells. In late proliferation stage embryos, two nuclei and then four nuclei, all at the embryo anterior, express the transgene. As the embryo begins morphogenesis, six nuclei, all at the very anterior pole, stain for beta-galactosidase activity. By the 1.5-fold stage, the embryo has eight staining nuclei, six at the anterior and two additional cells just ventral to the posterior bulb of the pharynx. By the time the embryo has completed morphogenesis, up to twelve staining nuclei can be detected. ceh-10/lacZ expression in L1 larvae is somewhat variable, in that staining in a particular cell is not always seen in every larva; the likely reason is that the level of ceh-10 mRNA declines after hatching and beta-galactosidase staining in L1 larvae presumably results from enzyme perdurance. Most ceh-10/lacZ staining occurs in the region around the nerve ring, which is dense with the nuclei and processes of neurons. The expressing anterior nuclei were identified as: AIYL/R (interneurons), CEPDL/R (mechanosensory neurons), RID (motor neuron), ALA (lateral neuron), RMED (nerve ring motor neuron), AINL/R (interneurons) and AVJL/R (ventral neurons). ceh-10 is also expressed in the CAN cells (the excretory Canal Associated Neuron).
• Primary Identifier: Expr1584

13 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Amphid interneuron	AIYL	lineage name: ABplpapaaap	WBbt:0003963
Amphid interneuron	AIYR	lineage name: ABprpapaaap	WBbt:0003961
Neuron class of one neuron, sends processes laterally and along dorsal cord.	ALA	lineage name: ABalapppaaa	WBbt:0003955
Neuron class of one ring motoneuron, projects along dorsal cord, innervates dorsal body muscle.	RID	lineage name: ABalappaapa	WBbt:0003938
Process runs along excretory canal, no synapses, essential for survival	CANL	lineage name: ABalapaaapa	WBbt:0004949
Process runs along excretory canal, no synapses, essential for survival	CANR	lineage name: ABalappappa	WBbt:0004947
Cephalic neurons, contain dopamine	CEPDR	lineage name: ABarpapaappa	WBbt:0004937
Cephalic neurons, contain dopamine	CEPDL	lineage name: ABplaaaaappa	WBbt:0004938
Ring motor neuron	RMED	lineage name: ABalapppaap	WBbt:0005027
Neuron, synapses like AVHL/R	AVJR	lineage name: ABalapppppa	WBbt:0003846
Neuron, synapses like AVHL/R	AVJL	lineage name: ABalapapppa	WBbt:0003847
Ring interneuron	AINR	lineage name: ABalaapaaar	WBbt:0003965
Ring interneuron	AINL	lineage name: ABalaaaalal	WBbt:0003967

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000435	ceh-10	W03A3.1	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004