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The differences in the expression patterns of Pmbr-1::gfp and mbr-1::gfp might be due to the following reasons: first, additional regulatory elements might exist in introns and 3$(B!l(B-UTR regions, which are not included in the former construct; and second, as the product of Pmbr-1::gfp diffuses throughout the cytoplasm, it might be difficult to detect low levels of GFP expression. |
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Expr3681
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In strains that carry Pmbr-1::gfp, GFP expression was observed in restricted sets of interneurons in the head ganglia: AIM, RIC, RIH (or RIR), RIF (or RIG), and a pair of interneurons tentatively identified as AIN. Expression was also observed in three interneurons of the tail ganglia: PVP and an interneuron tentatively identified as DVA or DVC. In contrast, mbr-1::gfp was expressed in several more neurons compared to Pmbr-1::gfp, including sensory neurons. Expression of mbr-1::gfp was also detected in some intestinal cells at the late embryonic stages as well as in vulval cells and some somatic gonadal cells at the L4 stage. mbr-1::gfp expression becomes detectable first around the early comma stage, which corresponds to the time just after the birth of most neurons. |
The MBR-1::GFP fusion protein was localized to the nuclei, consistent with the idea that MBR-1 functions as a transcription factor. |
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Expr15567
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Expr15571
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Expr15572
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Expr15573
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Expr15579
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Expr15586
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Expr15651
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Expr15652
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Expr15589
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Expr15591
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Expr15598
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Expr15604
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Expr15608
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Expr15611
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Expr15436
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unc-17(3k) |
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Expr15372
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr733
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Staining is seen in a set of 47 nuclei in fixed newly hatched first larval stage (L1). All stained cells are neurons. Hermaphrodite express in cells. RIH, RIR, PVR, IL2L/R, URYVL/R, RIPL/R, AIZL/R, FLPL/R, ADAL/R, RMGL/R, BPUL/R, PLML/R, ALML/R, ALNL/R, HSNL/R, URBL/R, NSML/R, URADL/R, IL2DL/R, I2L/R, IL2VL/R, URAVL/R, URXL/R, AIML/R, URYDL/R, PQR, PVM, SDQL/R, PVDL/R, PHCL/R, PLNL/R. Male cells express as in hermaphrodite except for HSNL/R which die, and show expression in CEMDL/R, CEMVL/R which die in hermaphrodites. Expression pattern is first determined in the Q lineage. Once expression has been initiated in a cell, it is maintained by that cell and all of its descendants in all cases except for SDQ. |
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Expr3672
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The nep-1::GFP construct was specifically expressed in the pharyngeal cells of transgenic animals, first detectable during late embryogenesis and remaining until adulthood. In some, but not all, F1 and F2 generation adult nematodes, GFP expression was detected in the single head interneuron RIH. |
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Expr10713
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A hst-3.1 reporter displayed limited expression during early embryogenesis, but was visible in the pharynx and body wall muscle by the embryonic threefold stage. During larval and adult stages, the reporter continued to be expressed in body wall muscle, vulval muscle and the pharynx. In addition, we detected expression in at least six neurons in the head, including the NSM, RIH, RIS, and an unidentified pair of neurons as well as some select epithelial cells. No obvious expression was seen in either AIY or HSN interneurons. Additional expression is observed in the coelomocytes (cc), the distal tip cell (dtc), and rectal cells (U,F/K, B AND Y/PDA). In adults, expression is predominantly seen in body wall muscles, vulva muscles, the muscle arms, the pharynx, and the rectal epithelium. |
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Expr15443
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Double staining with antisera against GFP and the epithelial junction protein JAM-1 (MH27) was performed to characterize embryonic expression in more detail. |
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Expr1639
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Epidermal cells in C. elegans are born on the dorsal side of the embryo, and the epidermal sheet migrates ventrally to enclose other tissues and anteriorly to cover the head. slt-1::GFP was first detectable just as the migrating epidermal cells completely covered the head and was expressed in hyp4, hyp1, and other epidermal cells. At the 2-fold stage of embryogenesis, expression spread beyond the anterior cap of cells to other anteriorly located cells, including the most anterior head muscle cells and some anterior socket cells, a set of glia associated with head sensilla. During the second half of embryogenesis, when most cells and axons in the developing nervous system are migrating, slt-1::GFP expression was concentrated in anterior cells and excluded from the middle of the body. Expression in anterior cells, including the anterior hyp cells, continued throughout larval stages and in the adult. Around the two-fold stage of embryogenesis, slt-1::GFP expression became evident in pharyngeal cells, including the most posterior pharyngeal muscle. Expression in the pharynx persisted through the L1 stage and in the adult. Beginning at the 2-fold stage of embryogenesis, slt-1::GFP was expressed in dorsal body wall muscles in a striking asymmetric pattern. Dorsal muscle expression began in the posterior embryo and spread anteriorly by the L1 larval stage, all dorsal muscles expressed slt-1::GFP. Robust expression in dorsal muscles persisted throughout the life of the animal. slt-1::GFP was also expressed in ventral body wall muscles, beginning in the L1 stage in posterior ventral muscles and continuing to more anterior muscles in later larval stages. Throughout development, dorsal muscles expressed more slt-1::GFP than ventral muscles. slt-1::GFP was expressed in the BDU neuron in the body and the RIH and RMED neurons, which have the most anterior axons in the nerve ring. Expression was first detected in comma stage embryos, with expression restricted to a cap of anterior cells. slt-1::GFP is not expressed in ventral epidermal cells. slt-1::GFP was expressed in the anal sphincter muscle. |
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Expr15648
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Expr15657
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Expr12601
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sox-2 expression is restricted to subsets of neuroblasts. sox-2 is expressed relatively late in nervous system development, in the progenitor of differentiated neurons, but not in earlier neuroectodermal cells. sox-2 is also expressed in some progenitors of non-neural tissue, in the head hypodermis and the arcade cells. sox-2 is expressed in several postembryonic blast cells that are generated in the embryo. These blast cells include the B, Y, F, U and K rectal epithelial cells and the seam cells along the body. Although expression of sox-2 is absent in the terminal neurons generated by these lineages, sox-2 expression extends beyond the blast cell stage [e.g. sox-2 expression is maintained in the V5 daughters (V5a and V5p) but is lost in the next division].sox-2 is expressed in the sensory neurons AWB, AWC, IL1, IL2, URA, URB, OLL, the interneurons AIM, AIN, AVK, RIH and the motor neuron class RME. The sox-2 fosmid reporter or sox-2 smFISH did not show any expression in the germ line or oocytes of young adult worms. Expression patterns obtained by smFISH were very similar to the ones observed with fosmid reporters. |
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Picture: Fig 6. |
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Expr8786
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Apart from cells in the neuroblast lineage that generate ASE, nhr-67::mCherry was expressed in multiple other neuroblast lineages in the developing embryo. Expression was usually observed in the grandmother or mother of a neuron, but not earlier. Within the ASEL and ASER-generating lineage branches, nhr-67 was expressed in neuroblasts that generate closely or distantly related cousins of ASEL and ASER. For example, the sister neuroblast of the ASE-generating neuroblast creates the AUA and ASJ neurons and it expressed nhr-67. The cousin of the ASE-mother cell generates the AWB and ADF sensory neurons. nhr-67 was expressed in these cells. In late stage embryos, a few other, postmitotic neurons started to express nhr-67. Embryonic nhr-67 expression was not restricted to the nervous system, but was observed in a small subset of mesodermal and hypodermal cells. No expression was detected in endodermal cells or the germ line. nhr-67 was expressed in the excretory canal cell. Postembryonically, nhr-67 expression persisted only in a few neurons in the head ganglia until the first larval stage and faded shortly thereafter in most, but not all, of these neurons, with expression persisting through adulthood only in the CEPD/V, RMED/V, AVL and RIS neurons. During mid-larval development, nhr-67 was transiently and dynamically expressed in the AC cells of the vulva. Expression was also found in the VU cells and somatic gonad, but not in vulA, vulB or vulC. Within the ASEL/R generating lineages, nhr-67::mCherry was first observed in the grandmother cells of ASEL and ASER. Transgenic animals that co-express a functional nhr-67::mCherry reporter and a functional che-1::yfp reporter revealed that nhr-67 precedes che-1 expression. nhr-67 expression was maintained in the ASEL and ASER neurons until the first larval stage after which it became undetectable, whereas che-1 expression was maintained throughout the life of the animal. In spite of its genetically deduced role in asymmetric gene expression in ASEL and ASER, nhr-67 expression is bilaterally symmetric in ASEL and ASER. |
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Expr12716
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Expr12717
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3.7 kb |
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Expr15327
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Data modified according to Shawn Lockery's expression pattern curations. |
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Expr328
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UNC-30 is found in the nuclei of the D neurons. IN L1 worms anti-UNC-30 stained the 6 DD neurons and in L2 worm, stained 6 DD neurons and 13 VD neurons. UNC-30 protein were maintained at low level during L3 and later stages.Anti-UNC-30 also stained a pair of nuclei in the preanal ganglion of L1 and L2 animals, corresponding to the position of PVP. In addition, four nuclei in the head of L1 and L2 are weakly stained, identified as ASGL, ASGR, RIH and RID. |
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