WormMine: ExpressionPattern Expr10616

• Pattern: ceh-19aprom::gfp fusion: no GFP expression was observed, throughout the normal life cycle or in the dauer, suggesting the ceh-19a transcript may not be functional or may only be expressed in circumstances not assayed. ceh-19bprom::gfp fusion: ceh-19 is expressed in MCL/R, ADFL/R. The GFP signal was much higher in MCs than in ADFs during normal developmental stages whereas the strength of signal in the two neuron types was approximately the same in the dauer stage due to the considerable increase of signal within ADFs. The two cells in the tail showing GFP expression have cell bodies located just behind the rectum and short processes in both anterior and posterior directions, a morphology suggestive of the phasmids neurons, PHAL/R or PHBL/R. DiI filling, which stains both of these phasmid neuron types revealing their relative positions, confirmed that the pair of neurons expressing GFP in the tail is the more anterior pair, PHAL/R. No male specific expression was observed for the ceh-19bprom::gfp fusion in any of the three transgenic strains.A fosmid containing 18,586 bp upstream and 15,366 bp downstream of ceh-19 showed consistent GFP expression in ADFL/R, MCL/R, and PHAL/R, as obtained with the promoter reporter fusion. GFP was distributed throughout the cell body and along the neuronal axon as expected for a transcriptional fusion, but the signal was lower than with the plasmid-based fusion. The recombineered C-terminal translational fusion, drove GFP expression in apparently the same pairs of neurons, i.e. ADFL/R, MCL/R, and PHAL/R, in 2 independent strains (UL3014, UL3308). GFP was localized to the cell nucleus only, as expected for a transcription factor fusion protein, and at very low level, making cell identification less certain. For both recombineered reporter fusions, expression in ADFL/R was very low through the normal life cycle but increased greatly in dauer animals.
• Primary Identifier: Expr10616

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Amphid neuron, prob. chemosensory	ADFL	lineage name: ABalpppppaa	WBbt:0004003
Amphid neuron, prob. chemosensory	ADFR	lineage name: ABpraaappaa	WBbt:0003999
Phasmid neurons, probably chemosensory	PHAL	lineage name: ABplpppaapp	WBbt:0004366
Phasmid neurons, probably chemosensory	PHAR	lineage name: ABprpppaapp	WBbt:0004365
Pharyngeal neurons that synapse onto marginal cells	MCL	lineage name: ABalpaaappp	WBbt:0004463
Pharyngeal neurons that synapse onto marginal cells	MCR	lineage name: ABarapaappp	WBbt:0004461

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000442	ceh-19	F20D12.6	Caenorhabditis elegans

0 Life Stages