WormMine: ExpressionPattern Expr1039

• Pattern: Antibody staining: CEH-17 expression was strong at the 3-fold to L1 stage and decreased thereafter to reach barely detectable levels in adults. At the L1 stage, CEH-17 was detected exclusively in the nuclei of five neurons of the ring ganglia. One of the five CEH-17+ neurons was dorsal and just posterior to the nerve ring, while the four others were ventral and close to the posterior bulb of the pharynx. pceh-17::GFP show that the dorsal neuron could be seen projecting two processes towards the ring, which then turned back and coursed along the lateral cords, all the way to the tail of the animal, identifying this neuron as ALA. The four ventral CEH-17 positive neurons were located just posterior to the SMBs, as was described for the SIAs. The four ventral ceh-17 expressing neurons are believed to be the SIA neurons (SIADR, SIADL, SIAVR and SIAVL). In addition to ALA and the four SIAs, a few other neurons also expressed the pceh-17::GFP construct. flceh-17::GFP staining resulted in the same expression pattern. ceh-17 HD::GFP showed effectively restricted GFP expression to the five neurons seen with the antibody (i.e. ALA and the four SIAs). The fluorescence had a granular appearance and was restricted to the cell body, possibly due to trapping of the chimeric protein in the endoplasmic reticulum. pceh-17::GFP::3 resulted in the same expression pattern (i.e. ALA and the four SIAs), but with axonal staining. The latter construct (pceh-17::GFP::3 ) was used to examine embryonic expression of ceh-17. At the comma stage, expression was detected in two dorsal neurons in the head, presumably corresponding to ALA and its sister RMED, and in six cells on the ventral side of the head, just anterior to the excretory pore. These cells probably correspond to the four SIAs, in which the GFP signal was strongest and revealed sprouting axons, and the two SIBVs, sisters of the SIADs. Two other weakly positive cells on the ventral side were detected, one in the mid-body region and one in the tail, at positions fitting those of motor neurons DB5 and DA8, sisters of the SIAVs. The earliest time at which could detect ceh-17::GFP expression was at the end of gastrulation in four pairs of cells, which could correspond to the four SIAs and their sisters or to the four SIA mothers and their own sisters. Since this is the time when the mothers of SIAs are undergoing their last division, it is not clear whether ceh-17 expression precedes or follows the last cell division.
• Primary Identifier: Expr1039
• Subcellular Localization: nuclei, cell body, axonal staining.

10 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Neuron class of one neuron, sends processes laterally and along dorsal cord.	ALA	lineage name: ABalapppaaa	WBbt:0003955
Ventral cord motor neurons, innervate dorsal muscles	DB5	lineage name: ABplpapappp	WBbt:0004842
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIBVL	lineage name: ABplpapaapp	WBbt:0004978
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIBVR	lineage name: ABprpapaapp	WBbt:0004977
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIAVL	lineage name: ABplpapappa	WBbt:0004985
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIAVR	lineage name: ABprpapappa	WBbt:0004983
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIADL	lineage name: ABplpapaapa	WBbt:0004989
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIADR	lineage name: ABprpapaapa	WBbt:0004987
Ring motor neuron	RMED	lineage name: ABalapppaap	WBbt:0005027
Ventral cord motor neurons, innervate dorsal muscles	DA8	lineage name: ABprpapappp	WBbt:0004858

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000440	ceh-17	D1007.1	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010
	A developemental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until death.		postembryonic Ce	WBls:0000022