No expression of GLF-1::GFP was seen in early embryogenesis. GFP first became visible at the four-fold embryonic stage, after completion of cuticle synthesis and approximately one hour before hatching. The signal was then seen throughout post-embryonic life. Expression was limited to the hypodermal cells. L4 larvae and adults showed much stronger expression of GLF-1::GFP in the seam cells as opposed to the main hypodermal syncytium and head and tail hypodermal cells.
Primary Identifier
Expr8792
Remark
Picture: Fig. 5.
Subcellular Localization
High resolution confocal micrographs of individual seam cells expressing the translational fusion indicates that GLF-1::GFP localizes to the cytoplasm.
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The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.
