WormMine: ExpressionPattern Expr8707

• Pattern: GFP was first observed in the descendants of the primordial germ cells in the early L1 larva. While GFP fluorescence was observed in the cytoplasm, stronger, punctuate fluorescence around the nucleus was unmistakable. This expression pattern continued through L2 and L3 stages. At L4 and adult stages, GFP expression was strongest in the mitotic germ cells and gradually decreased as the germ cells progressed through meiosis.
• Primary Identifier: Expr8707
• Remark: Picture: Fig. 1.
• Subcellular Localization: Many of the P granule-positive foci also contain PUF-8::GFP, indicating that PUF-8 is indeed localized on P granules.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004244	puf-8	C30G12.7	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024