1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006974 | zen-4 | M03D4.1 | Caenorhabditis elegans |
| Primary Identifier | Expr8629 | Remark | Picture: Figure 2A. To confirm the specificity of the phospho-CeMKLP1 antibody, authors performed immunofluorescence experiments on C. elegans embryos in the presence of cognate peptides. The phospho-CeMKLP1 antibody was preincubated either with phospho- or nonphosphopeptides. Phosphorylated ZEN-4 was detected at anaphase on the central spindle after preincubation with the nonphosphopeptide with no significant reduction of signal intensity, whereas preincubation of the phospho-CeMKLP1 antibody with the phosphopeptide abolished central spindle staining. |
| Subcellular Localization | The phospho-CeMKLP1 antibody shows a similar staining pattern. Phosphorylated ZEN-4 localizes to the anaphase spindle and persists into telophase, when midbody formation occurs. Aurora B and ZEN-4 have partially overlapping localization on the central spindle, and both become concentrated in the midbody and remnants. Thus, ZEN-4 is phosphorylated on the central spindle, midbody, and early remnant when it colocalizes with aurora B, consistent with ZEN-4 being an aurora B substrate. ZEN-4 localizes to a restricted region, which likely corresponds to the zone of microtubule overlap, on the central spindle in anaphase. It becomes concentrated in the midbody at telophase and persists at the remnant after sister-cell separation. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006974 | zen-4 | M03D4.1 | Caenorhabditis elegans |
