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Picture: Fig 3B to F. |
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Expr8610
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In transgenic animals, ARR-1::GFP was strongly expressed throughout the nervous system. ARR-1::GFP was expressed in the dorsal and ventral nerve cords and most or all neurons and their processes in the head and tail. Specifically, ARR-1::GFP was highly concentrated in the amphid chemosensory neurons, including the AWA, AWB, AWC, ADL, and ASH neurons. It was also observed in the HSN neuron. |
ARR-1::GFP in neuronal cells appeared to be primarily cytoplasmic and did not exhibit a distinct subcellular localization. |
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Expr1618
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A high proportion of PKC2 polypeptides is associated with the particulate fraction in newly-hatched (early L1) C. elegans and L3 and L4 larvae. |
In mid-L1 and L2 larvae, as well as young adult nematodes, PKC2 isoforms are nearly uniformly dispersed between cytosol and organelles and/or cytoskeleton. In contrast, 70% of PKC2 isoforms is isolated in cytosol from egg-laying adults. |
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Expr2635
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In addition to the components described above, adult males showed transgene neuronal expression in the developing and mature male tail. Independent lines of transgenic C. elegans expressing the reporter transgene showed fluorescence in embryo, larval and adult stages. Embryonic reporter gene activity was observed around the time of gastrulation and continued throughout development. Strong fluorescence was observed in the intestinal cells of larvae and adults, and this was particularly enhanced in the cells of the posterior gut. Larval and adult fluorescence was also observed in neuronal cell bodies around the nerve ring and in processes extending towards the tip of the nose, indicative of them being the amphid sensory neurons. |
Expressed in neuronal cell bodies and processes. |
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hda-4 was called hda-7 in this paper. |
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Expr1910
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GFP was expressed in several tissues of transgenic animals, including body-wall muscle cells, hypodermal seam cells, and neuronal cells including sensory amphid neuronal processes, the nerve ring, ventral nerve cords, and motor neuronal commissures. The expression in body-wall muscle was further confirmed by immunostaining using polyclonal antibodies against HDA-4. |
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Expr436
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Expression is predominantly neuronal and is seen from late embryo to adult. Neuronal expression consists of several nuclei of the head and tail ganglia, the ventral nerve cord, the circumpharyngeal nerve ring, several amphid processes and several processes within the tail spike. |
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