WormMine: ExpressionPattern Expr8980

• Pattern: GFP expression was observed at the larval and adult stages exclusively in all of the 20 intestinal cells of which a whole intestine consists. At the embryonic stage, no expression was found.
• Primary Identifier: Expr8980
• Remark: Picture: Fig 2, Fig 3, Fig S2A.
• Subcellular Localization: Double labeling of intestinal cells with acridine orange, a dye for acidic compartment staining. As a result, HAF-4::GFP-positive and acridine orange-positive granules turned out to be mutually exclusive, and the pattern was same in case of HAF-9:: GFP-positive granules. A similar exclusive pattern was observed when LysoTracker Red, another dye for monitoring the acidification of subcellular compartments, was used in place of acridine orange. In intestinal cells of the late larval and adult stages, HAF-4::GFP and HAF-9::GFP seemed, under confocal microscopy, to be distributed on the surface of intestinal granules of some sort. These results indicate that HAF-4 and HAF-9 are present on the membranes of some intestinal granules. The HAF-4::GFP-positive and HAF-9::GFP-positive granules were relatively large (>2 um average diameter) and accounted for the majority of distinguishable intestinal granules.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001814	haf-4	W04C9.1	Caenorhabditis elegans

0 Life Stages