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Expr12030
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Both YFP::LNKN-1 and LNKN-1::YFP are similarly localized to the plasma membrane of many cells. LNKN-1 begins to be expressed in all somatic gonadal cells of the male, including the LC, the vas deferens precursor cells, and seminal vesicle precursor cells, starting in the early L3 stage and continuing through adulthood. It is also expressed in all somatic gonadal cells of the hermaphrodite, including the distal tip cells, anchor cell, uterine precursor cells, and spermatheca precursor cells. Other expression occurs in pharynx, pharyngeal-intestinal valve, intestine, excretory cell and canal, seam cells, a specialized subset of hypodermal cells, the vulval precursor cells of the hermaphrodite, and hook precursor cells in the male. YFP-tagged LNKN-1 is localized to the plasma membrane, exhibiting stronger localization to the sides of cell-cell contact in tissues such as the intestine, seam, and gonad. |
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No detailed description on expression pattern in other life stages.. Picture: Fig. 1G, 1H. |
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Expr8874
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This reporter was expressed at low levels in the early somatic gonadal lineage. Prominent expression was first seen in Z1.pa and Z4.ap, and their descendants. In the L3 stage, the F12E12.5 reporter was expressed in ventral and dorsal uterine precursors. Reporter expression was not seen in early embryos, perhaps due to the limited sequences used to generate the reporter. |
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Expr9818
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GFP was observed in the M cell in the L1 and in all Mv cell daughters giving rise to the sex muscles in the L4. The GFP was occasionally maintained in vulval muscle cell nuclei into the adult. GFP expression was also seen in muscles in the head, in vulval and uterine precursors in L2s and L3s, and in several neurons in the head and ventral nerve cord from L2 to adult. There was also GFP expression in the intestine but this was inconsistent. (The images are of strain UL4037 which exhibited broader and stronger expression than UL3567, which had very weak GFP expression). |
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Expr16149
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Focusing on larval development, we found that LIN-12::mNG::3xFlag was expressed in all postembryonic mesoderm progenitor cells at the 4M and 8M stages, but was only localized to the nucleus in ventral M-lineage cells, consistent with functions in dorsoventral patterning of the M lineage (Foehr & Liu, 2008). LIN-12::mNG::3xFlag was subsequently visible in somatic gonad cells and in several PN.p cells where lin-12 regulates cell fates (reviewed by Sternberg, 2005). LIN-12::mNG::3xFlag was visible at the cell membrane and in internalized punctae in the P3.p - P8.p cells but localized to the nucleus only in P5.p and P7.p. Following vulval precursor cell patterning, LIN-12::mNG::3xFlag was highly expressed in numerous somatic gonad cells, with nuclear localization in several spermatheca and uterine precursor cells. |
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Expr12691
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CDC-42 is expressed broadly in the developing uterine and vulval cells, including the AC. |
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