For all transgenic worm lines, fluorescence was detected in the 20 intestinal cells and in the excretory cell. GFP expression was present at all larval stages and in adult worms, but was not found in embryos. Although no alteration in spatial and temporal expression was observed, the catp-5(6.2)::gfp construct led to a brighter fluorescence in all cells, when compared to worms that carried the catp-5(3.9)::gfp construct.
Primary Identifier
Expr8802
Remark
Picture: Fig. 2C, 2D.
Subcellular Localization
Inclusion of the entire catp-5 ORF and hence the complete set of predicted transmembrane helices in the catp-5(12.2)::gfp construct led to a membrane-bound GFP signal. Remarkably, the intestinal fluorescence was associated exclusively with the apical membrane that faces the gut lumen.
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2 Anatomy Terms
Definition
Name
Synonym
Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.
