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Picture: Figure 4G to 4R. |
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Expr8279
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FOZI-1 expression in the M lineage was first detectable in the nuclei of M.d and M.v at the 2-M stage (in 43% of the animals stained, n=21). This expression persisted through the next three rounds of cell divisions, such that all animals (n>50) at the 4-M through to the 16-M stage showed FOZI-1 expression in the M lineage. At the 16-M stage, two cells on the ventral side (M.vlpa and M.vrpa) divide one more time to produce two SMs and two BWM precursors and reach the 18-M stage. At the 18-M stage, FOZI-1 was still detectable in the undifferentiated BWMs and CCs, but not in the SMs (n=22). This expression of FOZI-1 at the 18-M stage appeared transiently and quickly became undetectable in the differentiated BWMs and CCs. FOZI-1 expression was not detected in the SM lineage. |
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CeTwist means hlh-8 here. See Expr607 for the pattern of the same locus. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr606
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Immuno fluorescence staining with antisera to ceTwist indicated similar expression pattern to reporter fusion. CeTwist first detected in L1 in defecation-associated muscles and in small number of neuron-like cells in the head. In later larvae, CeTwist was detected in SMs and their descendants. Expression in M and its descendants prior to to the SM stage was detected with reporter fusion but not with antibody. Expression in mature coelomocytes was also only detected with reporter fusions. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr607
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3-fold embryo staining first detected in M blast cell after it had finished its posterior migration. hlh-8::gfp detected after hatching in a set of rectum-associated cells that appeared to be defecation-associated muscles (this required full-length hlh-8 genomic fragment). During larval development hlh-8::gfp detected in M cell and all undifferentiated descendants of M. This expression was lost as cells differentiated into body wall or sex muscles. Expression was maintained in the two M derived coelomocytes throughout adult life. L2 expression was seen in all four non-M-derived embryonic coelomocytes. Expression was detected in embryonic coelomocytes after the birth of both postembryonic M-derived coelomocytes. Expression also observed in head cells, likely to be neuronal. hlh-8::lacZ fusion with addition 5' sequences showed reporter activity in coelomocytes. Expression in all these cells were nuclear localized both with gfp and lacZ. |
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Expr13686
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UNC-130::GFP expression is not detectable in the early M lineage from the 1-M to the 4-M stage. Beginning at the 8-M stage and continuing through the 18-M stage, UNC-130::GFP expression is visible in all the ventral, but not in any of the dorsal, M lineage cells. It was noticed that M.vlp and M.vrp and their descendants exhibited relatively higher levels of UNC-130::GFP expression than their anterior counterparts. |
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Expr13687
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UNC-130::GFP expression is not detectable in the early M lineage from the 1-M to the 4-M stage. Beginning at the 8-M stage and continuing through the 18-M stage, UNC-130::GFP expression is visible in all the ventral, but not in any of the dorsal, M lineage cells. It was noticed that M.vlp and M.vrp and their descendants exhibited relatively higher levels of UNC-130::GFP expression than their anterior counterparts. |
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