| Primary Identifier | Expr8557 | Remark | New Symbol: CABP14. Picture: Figure 5. |
| Subcellular Localization | CABP14 localizes to the actin-rich cortex of embryonic cells in a cell-cycle-dependent manner. It localizes to the nucleus of cells in prophase, to the nucleus and cortex of cells in prometaphase, to the cortex and cytoplasm of cells in metaphase and to the cleavage furrow of dividing cells. These various cell-cycle-dependent patterns are obvious from three different early embryos co-stained with CABP14 and actin antibodies. In the first embryo (two-cell stage), the posterior cell, P1, is in prophase and the anterior cell, AB, is in prometaphase. Typical of any embryonic cell in prophase, CABP14 is localized to the nucleus of P1 in this embryo and, typical of any embryonic cell in prmetaphase, CABP14 is localized predominantly to the nucleus and cortex of AB. In the second embryo (four-cell stage), the two daughters of AB (ABa and ABp) are in metaphase and the two daughters of P1 (EMS and P2) in prophase. The two metaphase cells show intense staining around the cortex of each cell and also some staining in the cytoplasm, whereas the two prophase cells show only nuclear staining. In the third embryo (eight-cell stage), P2 is dividing into C and P3. Localization of CABP14 along the cleavage furrow between the forming cells is evident. Two of the AB granddaughters, both in prometaphase, are in view and show nuclear and cortical CABP14 localization. The beginning of nuclear localization in the forming C and P3 cells and the recently divided E cell is faintly visible. CABP14 staining at different cleavage furrows varies from robust to weak, suggesting either a temporal component to its localization at the cleavage furrow or its association with a labile structure at the cleavage furrow. CABP14 staining is not detected in interphase or anaphase cells. |
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation. | blastula embryo Ce | WBls:0000005 |
