1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006976 | zhp-3 | K02B12.8 | Caenorhabditis elegans |
| Primary Identifier | Expr8515 | Remark | Picture: Figure 1. |
| Subcellular Localization | During early prophase, ZHP-3 protein localized along the synaptonemal complex (SC). The protein was initially detected on chromosomes in early pachytene. Although its localization to the SC required the loading of central element components, as previously reported, it initially appeared as puncta along continuous stretches of SC. This preceded complete synapsis, since ZHP-3 localized to synapsed chromosome regions in the presence of unsynapsed chromosomes, which did not stain with ZHP-3. ZHP-3 then accumulated along the SC so that by mid-pachytene, it had spread along the full length of the SC. However, by contrast to the axial and central element proteins (respectively) HTP-3 and SYP-1, its appearance was not uniform along the length of the SC. By late pachytene, localization along the length of the SC became more restricted, such that each synapsed homolog pair showed an extensive stretch of SYP-1 lacking ZHP-3. Three-dimensional reconstruction of pairs of homologs revealed that ZHP-3 was asymmetrically localized from an interstitial point to one end of the SC. The presence of SYP-1 along the full length of these synapsed chromosomes indicates that this shift in ZHP-3 localization precedes the crossover-dependent asymmetric disassembly of the SC that accompanies the transition from late pachytene to diplotene. Mutations that prevent complete synapsis and thereby extend the region of polarized nuclear morphology (e.g., pairing center deficiencies or him-8 mutants) also delayed the shift in ZHP-3 distribution, extending the region of nuclei that retain ZHP-3 localization along the length of the SC. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006976 | zhp-3 | K02B12.8 | Caenorhabditis elegans |
