WormMine: ExpressionPattern Expr8185

• Primary Identifier: Expr8185
• Remark: Picture: Fig. 1.
• Subcellular Localization: Anti-IFB-2 fluorescence was detectable diffusely in the cytoplasm of intestinal cells accumulating over time in the sub-apical domain next to the newly forming lumen (e.g., plum stage). Anti-IFC-2 antibodies reacted only very weakly with the intestinal cells at plum stage although co-distribution with anti-IFB-2 was detectable in some sub-apical areas. The major IFC-2 immunofluorescence, however, was seen in hypodermal cells presenting a multipunctate staining of cell borders indicating a junctional localization. In subsequent stages, anti-IFB-2 reactivity further concentrated in the periluminal compartment, i.e., in the electron-dense endotube ultrastructure. Strongest immunofluorescence was noted in junctional regions due to the localization of IFB-2 along the entire junctional complex. In addition, multiple cytoplasmic puncta were seen that had not been described previously. These fluorescent structures began to accumulate in L3-larvae and were most abundant in old adults with a predilection for the posterior intestine. The anti-IFC-2-staining gradually increased in the intestine becoming quite prominent in L3-larvae in which it presented a very similar distribution to anti-IFB-2 fluorescence although diffuse cytoplasmic and junctional staining were more pronounced. In adult intestines anti-IFC-2 fluorescence was essentially identical to that of anti-IFB-2.

0 Anatomy Terms

2 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002054	ifb-2	F10C1.7	Caenorhabditis elegans
WBGene00002056	ifc-2	M6.1	Caenorhabditis elegans

0 Life Stages