WormMine: ExpressionPattern Expr15183

• Pattern: We examined cnd-1p::mCherry expression in early L1 larvae, within an hour of hatching, counting nuclei in the retrovesicular ganglion and also the ventral nerve cord. The retrovesicular ganglion is a linear cluster of cells located on the ventral midline of the worm immediately posterior to the pharynx, and contains the anterior most DA, DB and DD motorneurons (DA1, DB1, DB2 and DD1), along with eight additional cells. Wild type animals showed an average of nine cnd-1p::mCherry nuclei in the retrovesicular ganglion and 10 in theventral nerve cord (n = 26). As there are 22 motorneurons in early L1 larvae, this suggests that only a subset express the cnd-1 reporter gene. Based on their location along the ventral nerve cord, co-labeling with unc-25p::GFP (a known DD neuron marker), and by corroborating against single cell RNA-seq expression data (Packer et al. 2019), we tentatively conclude that cnd-1p::mCherry is expressed in DA1-5, DB1, DB3, and DD1-6.
• Primary Identifier: Expr15183

15 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
Ganglion that lies at the anterior limit of the ventral nerve cord, near the ventral ganglion and nerve ring in the head posterior to the excretory pore. It is open and continuous with the region containing the motoneurons of the ventral cord. In the early L1 this ganglion holds 12 neuron cell bodies plus one neuroblast (Sulston and Horvitz, 1977; White et al., 1986). In the adult animal, the ganglion holds 20 neuron cell bodies.	retrovesicular ganglion		WBbt:0005656
Ventral cord motor neurons, innervate dorsal muscles	DB3	lineage name: ABprpaaaapp	WBbt:0004844
Ventral cord motor neurons, innervate dorsal muscles	DA2	lineage name: ABplppapapa	WBbt:0004869
Ventral cord motor neurons, innervate dorsal muscles	DA3	lineage name: ABprppapapa	WBbt:0004867
Ventral cord motor neurons, innervate dorsal muscles	DA4	lineage name: ABplppapapp	WBbt:0004865
Ventral cord motor neurons, innervate dorsal muscles	DA5	lineage name: ABprppapapp	WBbt:0004863
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD6	lineage name: ABprppapppp	WBbt:0004824
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD2	lineage name: ABprppappap	WBbt:0004838
Ventral cord motor neurons, innervate dorsal muscles	DA1	lineage name: ABprppapaap	WBbt:0004871
Ventral cord motor neurons, innervate dorsal muscles	DB1	lineage name: ABplpaaaapp	WBbt:0004846
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD4	lineage name: ABprppapppa	WBbt:0004826
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD5	lineage name: ABplppapppp	WBbt:0004825
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD3	lineage name: ABplppapppa	WBbt:0004837
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD1	lineage name: ABplppappap	WBbt:0004839

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000561	cnd-1	C34E10.7	Caenorhabditis elegans

0 Life Stages