WormMine: ExpressionPattern Expr2942

• Pattern: WRN-1 was immunolocalized in C. elegans at various developmental stages. The protein was non-uniformly distributed in nuclei from the early embryonic stage throughout embryogenesis. In early embryos, the protein level was higher in mitotic cells than in interphase cells, as is clearly shown in 6- and 8-cell stage embryos. In larval stages WRN-1 was present in the nuclei of numerous cells, and the fraction of WRN-1-positive somatic cells was significantly lower in adults. In adult worms, the protein was expressed in hypodermal, intestinal and germ cells, and the protein level decreased with age.
• Primary Identifier: Expr2942
• Remark: RNAi of wrn-1 eliminated immunologically detectable protein in embryos, supporting effective suppression of the endogenous gene expression and no cross-reactivity of the antibody. By contrast, RNAi of a RecQ homolog (RCQ-5) encoded by the E03A35.2 ORF, with 39% amino acid identity in the helicase domain of WRN-1, did not affect WRN-1 expression, thus underlining the specificity of wrn-1 RNAi.
• Subcellular Localization: At metaphase, WRN-1 had an unusual location, overlapping with the periphery of equatorially aligned condensed chromosomes facing the spindle poles, and also, less frequently, overlapping with the mitotic spindles. When nuclei in the intestine were magnified, an uneven distribution of the protein was observed. Both mitotic and meiotic prophase germ cells in the L4 stage gonad contained WRN-1 in the nuclei, but the level was significantly reduced in the meiotic prophase germ cells of adults. In the oocytes of adult gonads, WRN-1 localized to condensed chromosomes.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
any of 20 large epithelial cells which form a tube and are mostly situated as bilaterally symmetric pairs around the tubular lumen. Each of these cell pairs forms an intestinal ring ( II-IX int rings). The most anterior intestinal ring (int ring I), however, is made of four cells. Intestinal cells contain large nuclei with large nucleoli and numerous autofluorescent granules in their cytoplasm.	intestinal cell		WBbt:0005792

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006944	wrn-1	F18C5.2	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003