WormMine: ExpressionPattern Expr2664

• Pattern: Expressed in intestinal cell.
• Primary Identifier: Expr2664
• Remark: Gene_regulation: MTM-6 contains a putative amino-terminal GRAM domain, a PTP catalytic domain, a set interaction domain (SID), and a carboxyl-terminal FYVE domain. The GRAM domain is a novel 70 amino acid domain located at the amino terminus of several MTMs and has been proposed to act as an intracellular protein or lipid-binding signaling module. The SID was identified initially on the basis of its interaction with the SET domain of the Hrx protooncogene and might also mediate protein-protein interactions. Although both MTM-6 and MTM-1 contain putative GRAM and SID domains, only MTM-6 contains a FYVE domain, suggesting that this domain might target MTM-6 to the plasma membrane. However, neither the GRAM nor the FYVE domains are required for apical targeting as MTM-6-GFP fusion proteins lacking either the FYVE or the GRAM domain target to apical membrane. The role the PTP or SID domains could not be assessed because mutants that lack either the PTP or SID domains are not expressed, presumably because this results in the production of an unstable protein.
• Subcellular Localization: MTM-6-GFP fusion protein was observed at high levels in a uniform pattern associated with the apical membrane of the intestinal cell. By contrast, expression of GFP alone, or a GFP fusion protein containing only the MTM-6 FYVE domain, exhibited a diffuse cytosolic expression in the intestine.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
any of 20 large epithelial cells which form a tube and are mostly situated as bilaterally symmetric pairs around the tubular lumen. Each of these cell pairs forms an intestinal ring ( II-IX int rings). The most anterior intestinal ring (int ring I), however, is made of four cells. Intestinal cells contain large nuclei with large nucleoli and numerous autofluorescent granules in their cytoplasm.	intestinal cell		WBbt:0005792

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003478	mtm-6	F53A2.8	Caenorhabditis elegans

0 Life Stages