WormMine: ExpressionPattern Expr2570

• Pattern: Examination of male and hermaphrodite worms possessing the T03F1.5 pro::GFP construct demonstrated consistent GFP expression in two different anatomical regions. The first region identified was at the posterior end of the intestine of both hermaphrodite and male worms at all larval stages, and was consistent with the location of the sphincter muscle. The second region was at the anterior end of both hermaphrodite and male worms. Two cells anterior to the first pharyngeal bulb were linked to consistent GFP expression from the L1 to the adult stages. The two cell bodies located in the hypodermis on the dorsal and ventral side of the 'head' appeared to contain cellular processes which extended to the anterior end of the worm, in a fashion similar to dendrites. These processes bifurcated toward the anterior end, with the dorsal and ventral branches predicted to terminate in sensillae near the surface. Comparison of the observed expression patterns and the anatomical 'map' of C. elegans hermaphrodites indicated that no neurons are localised to this region, suggesting that the cells were not of this category. Instead, the GFP expression was consistent with two neuron-associated cells, namely the inner labial socket cell dorsal left (IlsoDL) and inner labial socket cell ventral left (IlsoVL). Transgenic worms which contained the pc::GFP (promoter and coding sequence) constructs showed similar expression profiles to those containing the promoter alone, but the GFP expression was significantly reduced.
• Primary Identifier: Expr2570

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
a saddle-shaped muscle cell that encircles the intestinal-rectal valve	anal sphincter muscle	lineage name: ABprpppppap	WBbt:0005798
Inner labial socket	ILsoDL	lineage name: ABplaapaaap	WBbt:0004521
Inner labial socket	ILsoVL	lineage name: ABalppapapp	WBbt:0004503

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00020187	gsp-4	T03F1.5	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024